During mitotic prohase, cohesins are removed from the chromosome arms by WAPP to ensure a faithful separation of the Chromatide sister. However, during feminine meiosis I, the resolution of chiasmata requires the proteolytic cleavage of the REC8 cohesin subunit along the chromosome arms by Separase to separate the counterparts and that the role of WAPP remained unknown. Here we note that Wapul works as a spindle assembly control point regulator (bag) to prevent aneuploidy in meiosis I. Wapl exhaustion accelerates meiotic progression, inactive bag and causes mediotic defects such as That the structure of the aberrant spindle / chromosomal and the incorrect kinechore microtubule (K-MT) attachment, therefore resulting in aneuploid eggs. In particular, we identify Bub3 as a binding partner of Waple by immunoprecipitation and analysis of mass spectrometry.

We also determine that WAPP controls the activity of the bag by maintaining the BUB3 protein level and document that BUB3 exogenous restores normal meiosis of exhausted Wapl. Together, our conclusions discover unique and non-painted roles for Wapl in the control of the mediation of the bag in female meiosis I. Cohesic is essential to the folding and heritage of the genome. In somatic cells, these functions are both mediated by SCC1-cohesin, which in mitosis is released from chromosomes by WAPP and separated. In mammal oocytes, cohesion is mediated by REC8-cohesin. SCC1 is expressed, but neither required nor sufficient for cohesion, and its function remains unknown.

Similarly, it is unclear whether WAPP regulates one or the cohesic complexes and segregation of chromosomes in mature oocytes. Here, we show that WAPP is required for accurate chromosomal segregation of meiosis I, mainly releases chromosome SCC1 cohesic and promotes the production of eggs employed. Using a single HI-C kernel, we found that CSC1 is essential for chromosomal organization in oocytes.

 

Functions closely separated and caspase in cell division and programmed cell death.

The separate chromatide separation, promoted by sepaase, is essential to the faithful chromosomal segregation. Sepase is a member of CYSTEINE CD CLAN CD, which also includes pro-apoptotic enzymes called Caspases. We signal a role for the C. elegans separated Sep-1, mainly known for its essential activity in cell division and the cortical granule exocytosis, in the cell death programmed for development when the predominant pro-apoptotic caspase ceded-3 is compromised. The loss of SEP-1 gives additional surviving cells in a low CED-3 (-) mutant and removes the embryonic lethality of a defective mutant for the CED-9 / BCL-2 apoptotic suppressor involving Sep-1 in the ‘Execution of apoptosis.

We also state non-apoptotic apparent roles for CED-3 in promoting the proliferation of germ cells, the méiotic chromosomal disjunction, the formation of egg shells and the normal rate of embryonic development. In addition, the loss of CASPase inhibitors specific to SOMA (CSP-3) and gerine-specific (CSP-2) results in a dependent suppression of ECD-3 embryonic lethality and méiotic chromosomal non-disjunction , when the separase function is compromised.

Thus, while caspses and sepaases have evolved from different substrate specificities associated with their specialized duties in apoptosis and cell division, respectively, they seem to have kept the residual capacity to participate in the two processes, supporting the opinion that The co-option of components in the cell division may have led to the innovation of the cell suicide scheduled early in the evolution of the metazoan.

Data is set on toxicity inhibitor separator-1 Sepin-1 in the weight of organs, hematology and clinical parameters in the Sprague-Dawley mouse.

Sepease inhibitors-1-1 has shown a big promise as a development chemotherapy agent to treat separate tumors, however, very little known about the toxicity profile. Here we present a data collection of organ weight, hematology, and clinical chemical parameters in Sprague-Dawley mice treated with 1. Data sets resulting from two study groups – Main studies and recovery studies, with a lifetime duration of 29 and 57 days respectively , Mice in both groups taxated with 0, 5, 10 and 20 mg / kg Sepin-1 once a day for 28 consecutive days. Blood samples from mice in the main studies were collected and their organs were weighed on the 29th day.

Animals in recovery studies are in the dose period of 28 days after destined to be sepilin-1 for 28 days, and their weight and weight data is collected on day 57. The body weight of rats in the main study and recovery are collected twice a week. Hematology parameters of intact blood samples, such as hemoglobin concentrations, platelet counts and blood cells etc., determined. Serum clinical chemical parameters, such as albumin concentration, glucose, cholesterol, triglycerides, alanine / aspartate aminotransferases, etc., are measured. Further analysis can produce useful information about 1-1 toxicity in the Sprague-Dawley mouse. The data presented here is related to the study article entitled “Study of Sepoin-1 Inhibitor toxicity in Sprague-Dowley Raths”, available in pathology – journal research and practice.

N-Acetyl-L-cysteine
GM8803-500G	Glentham Life Sciences	500 g	EUR 170.4

N-Acetyl-L-cysteine
B1813-1G	Biovision	each	EUR 144

N-Acetyl-L-cysteine
B1813-5G	Biovision	each	EUR 392.4

N-Acetyl-S-phenyl-L-cysteine
GM3549-10MG	Glentham Life Sciences	10 mg	EUR 104.4

Human Lipopolysaccharide-responsive and beige-like anchor protein (LRBA)
1-CSB-EP013070HU(N)	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Lipopolysaccharide-responsive and beige-like anchor protein(LRBA),partial expressed in E.coli

Human Thrombospondin-1 (THBS1)
1-CSB-RP073494h(N)	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Thrombospondin-1(THBS1) ,partial expressed in E.coli

Human Breast cancer type 1 susceptibility protein (BRCA1)
1-CSB-RP096594h(N)	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Breast cancer type 1 susceptibility protein(BRCA1),partial expressed in E.coli

Human Transcription termination factor 1 (TTF1)
1-CSB-RP116774h(N)	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Transcription termination factor 1(TTF1),partial expressed in E.coli

Helicobacter pylori Vacuolating cytotoxin autotransporter (vacA)
1-CSB-RP143774Ba(N)	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Helicobacter pylori Vacuolating cytotoxin autotransporter(vacA),partial expressed in E.coli

Rat Vesicle-fusing ATPase (Nsf)
1-CSB-RP154574r(N)	Cusabio	EUR 606.00 EUR 318.00 EUR 2192.40 EUR 919.20 EUR 1461.60 EUR 402.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rat Vesicle-fusing ATPase(Nsf),partial expressed in E.coli

Saccharomyces cerevisiae Trehalose-phosphatase (TPS2)
1-CSB-RP165694Ye(N)	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Saccharomyces cerevisiae Trehalose-phosphatase(TPS2),partial expressed in E.coli

Human Histone deacetylase 7 (HDAC7)
1-CSB-RP178994h(N)	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Histone deacetylase 7(HDAC7),partial expressed in E.coli

Human Telomerase protein component 1 (TEP1)
1-CSB-RP180494h(N)	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Telomerase protein component 1(TEP1),partial expressed in E.coli

Saccharomyces cerevisiae Neutral trehalase (NTH1)
1-CSB-RP181594Ye(N)	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Saccharomyces cerevisiae Neutral trehalase(NTH1),partial expressed in E.coli

Saccharomyces cerevisiae Acyl-CoA-binding protein (ACB1)
1-CSB-YP006519SVG(N)	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Saccharomyces cerevisiae Acyl-CoA-binding protein(ACB1) expressed in Yeast

Human Nuclear pore membrane glycoprotein 210 (NUP210)
1-CSB-EP016195HU(N)	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Nuclear pore membrane glycoprotein 210(NUP210),partial expressed in E.coli

N-Acetyl-L-cysteine (>99%, cell culture grade)
NALC15-1	Alpha Diagnostics	1 g	EUR 270

N-Acetyl-L-cysteine (>99%, cell culture grade)
NALC15-5	Alpha Diagnostics	5 g	EUR 489.6

N-Acety-L-Cysteine
abx082583-25g	Abbexa	25 g	EUR 243.6

N-acetyl-L-alanine
20-abx184259	Abbexa	EUR 410.40 EUR 292.80 EUR 243.60	100 g 25 g 5 g

N-Acetyl-L-tyrosine
HY-W012382	MedChemExpress	100mg	EUR 129.6

N-Acetyl-L-phenylalanine
GM0143-100G	Glentham Life Sciences	100 g	EUR 274.8

N-Acetyl-L-phenylalanine
GM0143-25G	Glentham Life Sciences	25 g	EUR 132

N-Acetyl-L-phenylalanine
GM0143-5G	Glentham Life Sciences	5 g	EUR 69.6

N-Acetyl-L-tyrosine
GM0216-100G	Glentham Life Sciences	100 g	EUR 84

N-Acetyl-L-tyrosine
GM0216-1KG	Glentham Life Sciences	1 kg	EUR 228

N-Acetyl-L-tyrosine
GM0216-250G	Glentham Life Sciences	250 g	EUR 112.8

N-Acetyl-L-tyrosine
GM0216-25G	Glentham Life Sciences	25 g	EUR 60

N-Acetyl-L-tyrosine
GM0216-500G	Glentham Life Sciences	500 g	EUR 151.2

N-Acetyl-L-methionine
GM6577-25G	Glentham Life Sciences	25 g	EUR 103.2

N-Acetyl-L-tryptophan
GM7304-100G	Glentham Life Sciences	100 g	EUR 189.6

N-Acetyl-L-tryptophan
GM7304-10G	Glentham Life Sciences	10 g	EUR 64.8

N-Acetyl-L-tryptophan
GM7304-25G	Glentham Life Sciences	25 g	EUR 86.4

N-Acetyl-L-tryptophan
GM7304-50G	Glentham Life Sciences	50 g	EUR 122.4

N-Acetyl-L-tryptophan
GM7304-5G	Glentham Life Sciences	5 g	EUR 54

N-Acetyl-L-carnosine
GM8692-5G	Glentham Life Sciences	5 g	EUR 180

N-Acetyl-L-leucine
HY-59291	MedChemExpress	500mg	EUR 129.6

L-Cysteine
CB0132	Bio Basic	100g	EUR 84.01

L-Cysteine
20-abx082146	Abbexa	EUR 276.00 EUR 226.80	100 g 25 g

L-Cysteine
20-abx082309	Abbexa	EUR 326.40 EUR 243.60	100 g 25 g

L-Cysteine
HY-Y0337	MedChemExpress	1g	EUR 142.8

L-Cysteine
GM3760-100G	Glentham Life Sciences	100 g	EUR 64.8

L-Cysteine
GM3760-1KG	Glentham Life Sciences	1 kg	EUR 170.4

L-Cysteine
GM3760-250G	Glentham Life Sciences	250 g	EUR 84

L-Cysteine
GM3760-25G	Glentham Life Sciences	25 g	EUR 55.2

L-Cysteine
GM3760-500G	Glentham Life Sciences	500 g	EUR 112.8

N-Acetyl-L-aspartic acid
GM8485-1G	Glentham Life Sciences	1 g	EUR 64.8

N-Acetyl-L-aspartic acid
HY-113524	MedChemExpress	10mM/1mL	EUR 135.6

N-Acetyl-3,5-dinitro-L-tyrosine
20-abx185512	Abbexa	EUR 360.00 EUR 811.20	1 g 5 g

L-Cysteine (hydrochloride)
HY-Y0337A	MedChemExpress	1g	EUR 142.8

Acetyl-Pepstatin
N-1250.0001	Bachem	1.0mg	EUR 211.2
Description: Sum Formula: C31H57N5O9; CAS# [28575-34-0]

Acetyl-Pepstatin
N-1250.0005	Bachem	5.0mg	EUR 733.2
Description: Sum Formula: C31H57N5O9; CAS# [28575-34-0]

N-Acetyl-L-tyrosine ethyl ester monohydrate
GM5614-10G	Glentham Life Sciences	10 g	EUR 156

N-Acetyl-L-tyrosine ethyl ester monohydrate
GM5614-5G	Glentham Life Sciences	5 g	EUR 103.2

REAGENT BOTTLE, 2 L, PP
1500P-2L	CORNING	6/pk	EUR 94.8
Description: Reusable Plastics; Resusable Bottles

L-Cysteine hydrochloride, monohydrate
CB0133	Bio Basic	100g	EUR 78.79

L-Cysteine [G-BSA]
DAG3280	Creative Diagnostics	250ug	EUR 1450.8

L-Cysteine hydrochloride monohydrate
20-abx082292	Abbexa	EUR 292.80 EUR 226.80	100 g 25 g

L-Cysteine Thylester Hydrochloride
abx188747-1kg	Abbexa	1 kg	EUR 760.8

S-Allyl-L-Cysteine
abx188873-250g	Abbexa	250 g	EUR 1178.4

L-Cysteine Monoclonal, 50UG
A002-50UG	Arbor Assays	50UG	EUR 315.6

S-Allyl-L-cysteine
HY-W013573	MedChemExpress	10mM/1mL	EUR 135.6

S-Benzyl-L-cysteine
GM1431-25G	Glentham Life Sciences	25 g	EUR 98.4

L-Cysteine hydrochloride, anhydrous
GM1539-100G	Glentham Life Sciences	100 g	EUR 55.2

L-Cysteine hydrochloride, anhydrous
GM1539-1KG	Glentham Life Sciences	1 kg	EUR 132

L-Cysteine hydrochloride, anhydrous
GM1539-250G	Glentham Life Sciences	250 g	EUR 69.6

L-Cysteine hydrochloride, anhydrous
GM1539-500G	Glentham Life Sciences	500 g	EUR 93.6

L-Cysteine hydrochloride monohydrate
GM4222-100G	Glentham Life Sciences	100 g	EUR 55.2

L-Cysteine hydrochloride monohydrate
GM4222-1KG	Glentham Life Sciences	1 kg	EUR 122.4

L-Cysteine hydrochloride monohydrate
GM4222-250G	Glentham Life Sciences	250 g	EUR 69.6

L-Cysteine hydrochloride monohydrate
GM4222-500G	Glentham Life Sciences	500 g	EUR 84

S-Methyl-L-cysteine
HY-B2188	MedChemExpress	100mg	EUR 142.8

n-Heptane Reagent
HC5400	Bio Basic	1L	EUR 94.8

L-Fucose
FUC15-N	Alpha Diagnostics	1 g	EUR 578.4

Acetyl-L-homoserine lactone
A147-25MG	TOKU-E	25 mg	EUR 295.2

Acetyl-L-homoserine lactone
A147-5MG	TOKU-E	5 mg	EUR 110.4

Nepsilon-Acetyl-L-lysine
HY-113426	MedChemExpress	100mg	EUR 129.6

4 L REAGENT GRADE WATER, CUBE
46-002-LF	CORNING	4 L/pk	EUR 109.2
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

Total Chlorine Reagent 0-2.50mg L
HI9370103	Scientific Laboratory Supplies	PK300	EUR 94.62

Total Chlorine Reagent 0-3.50mg L
HI9371103	Scientific Laboratory Supplies	PK300	EUR 95.76

Ammonia HR 0 -100mg/l reagent
WAT1048	Scientific Laboratory Supplies	EACH	EUR 103.74

L-Cysteine ethyl ester hydrochloride
GM3732-100G	Glentham Life Sciences	100 g	EUR 180

L-Cysteine ethyl ester hydrochloride
GM3732-25G	Glentham Life Sciences	25 g	EUR 74.4

L-Cysteine methyl ester (hydrochloride)
HY-B1038	MedChemExpress	1g	EUR 142.8

COD LR 0 to 150mg/L reagent
WAT1030	Scientific Laboratory Supplies	EACH	EUR 64.98

COD MR 0 to 1500mg/L reagent
WAT1032	Scientific Laboratory Supplies	EACH	EUR 64.98

COD HR 0 to 15000mg/L reagent
WAT1034	Scientific Laboratory Supplies	EACH	EUR 64.98

S-Sulfo-L-cysteine sodium salt
B6211-10	ApexBio	10 mg	EUR 439.2

S-Sulfo-L-cysteine sodium salt
B6211-50	ApexBio	50 mg	EUR 1654.8

N-Acetyl-Calicheamicin
HY-19791	MedChemExpress	10mM/1mL	EUR 30834

L-trans-Epoxysuccinyl-Leu-4-guanidinobutylamide
N-1645.0005	Bachem	5.0mg	EUR 297.6
Description: Sum Formula: C15H27N5O5; CAS# [66701-25-5]

L-trans-Epoxysuccinyl-Leu-4-guanidinobutylamide
N-1645.0025	Bachem	25.0mg	EUR 1082.4
Description: Sum Formula: C15H27N5O5; CAS# [66701-25-5]

L-trans-Epoxysuccinyl-Leu-3-methylbutylamide
N-1655.0001	Bachem	1.0mg	EUR 211.2
Description: Sum Formula: C15H26N2O5; CAS# [76684-89-4]

L-trans-Epoxysuccinyl-Leu-3-methylbutylamide
N-1655.0005	Bachem	5.0mg	EUR 733.2
Description: Sum Formula: C15H26N2O5; CAS# [76684-89-4]

EP Reagent Biuret Reagent
1011601	Scientific Laboratory Supplies	1L	EUR 42.18

EP Reagent Iodoplatinate Reagent
1046300	Scientific Laboratory Supplies	200ML	EUR 360.24

EP Reagent Methoxyphenylacetic Reagent
1053601	Scientific Laboratory Supplies	100ML	EUR 354.54

EP Reagent Molybdovanadic Reagent
1056700	Scientific Laboratory Supplies	100ML	EUR 42.18

EP Reagent Phosphomolybdotungstic Reagent
1065000	Scientific Laboratory Supplies	100ML	EUR 161.88

L-trans-Epoxysuccinyl-Ile-Pro-OH propylamide
N-1475.0001	Bachem	1.0mg	EUR 254.4
Description: Sum Formula: C18H29N3O6; CAS# [134448-10-5]

L-trans-Epoxysuccinyl-Ile-Pro-OH propylamide
N-1475.0005	Bachem	5.0mg	EUR 907.2
Description: Sum Formula: C18H29N3O6; CAS# [134448-10-5]

L-trans-Epoxysuccinyl-Ile-Pro-OMe propylamide
N-1660.0001	Bachem	1.0mg	EUR 254.4
Description: Sum Formula: C19H31N3O6; CAS# [147859-80-1]

L-trans-Epoxysuccinyl-Ile-Pro-OMe propylamide
N-1660.0005	Bachem	5.0mg	EUR 907.2
Description: Sum Formula: C19H31N3O6; CAS# [147859-80-1]

Cathepsin L, Human Liver
CTHL22-N-25	Alpha Diagnostics	25 ug	EUR 416.4

Claudin-18 Isoform 2 CHO Cell Line-78533-L
78533-L	BPS Bioscience	2 vials	EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full length human claudin 18.2 (claudin 18 isoform 2, CLDN 18.2, gene accession number NM_001002026.3). The stable clonal cell line was selected for different levels of Claudin 18.2 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.

N-acetyl Calicheamicin g1
ADC-P-117	Creative Biolabs	unit	Ask for price

N-Acetyl-D-glucosamine
AB2245	Bio Basic	25g	EUR 74.1

N-Acetyl-DL-alanine
abx184257-100g	Abbexa	100 g	EUR 326.4

N-acetyl D-galactosamine
B7904-100	ApexBio	100 mg	EUR 129.6

N-acetyl D-galactosamine
B7904-500	ApexBio	500 mg	EUR 199.2

N-Acetyl-DL-methionine
HY-W019704	MedChemExpress	100mg	EUR 129.6

N-Acetyl-D-mannosamine
GK3319-10G	Glentham Life Sciences	10 g	EUR 180

N-Acetyl-D-mannosamine
GK3319-1G	Glentham Life Sciences	1 g	EUR 73.2

N-Acetyl-D-mannosamine
GK3319-5G	Glentham Life Sciences	5 g	EUR 117.6

N-Acetyl-D-galactosamine
GK3532-100MG	Glentham Life Sciences	100 mg	EUR 79.2

N-Acetyl-D-galactosamine
GK3532-1G	Glentham Life Sciences	1 g	EUR 187.2

N-Acetyl-D-galactosamine
GK3532-500MG	Glentham Life Sciences	500 mg	EUR 132

N-Acetyl-D-alanine
GM2947-5G	Glentham Life Sciences	5 g	EUR 160.8

N-Acetyl-DL-tryptophan
GM4354-100G	Glentham Life Sciences	100 g	EUR 122.4

N-Acetyl-DL-tryptophan
GM4354-250G	Glentham Life Sciences	250 g	EUR 208.8

N-Acetyl-DL-tryptophan
GM4354-25G	Glentham Life Sciences	25 g	EUR 69.6

N-Acetyl-DL-tryptophan
GM4354-5G	Glentham Life Sciences	5 g	EUR 52.8

N-Acetyl-DL-valine
GM7740-25G	Glentham Life Sciences	25 g	EUR 112.8

N-Acetyl-D-glucosamine
GC7987-100G	Glentham Life Sciences	100 g	EUR 122.4

N-Acetyl-D-glucosamine
GC7987-250G	Glentham Life Sciences	250 g	EUR 184.8

N-Acetyl-D-glucosamine
GC7987-25G	Glentham Life Sciences	25 g	EUR 69.6

N-Acetyl-D-glucosamine
GC7987-500G	Glentham Life Sciences	500 g	EUR 274.8

N-Acetyl-D-glucosamine
GC7987-5G	Glentham Life Sciences	5 g	EUR 51.6

N-Acetyl-D-glucosamine
HY-A0132	MedChemExpress	10mM/1mL	EUR 135.6

N-acetyl Dapsone D4
HY-G0016S	MedChemExpress	10mg	EUR 3036

N-acetyl Dapsone (D4')
HY-G0016S1	MedChemExpress	1mg	EUR 472.8

N-Acetyl-5-hydroxytryptamine
HY-107854	MedChemExpress	10mM/1mL	EUR 181.2

N-Acetyl-D-mannosamine
HY-128850	MedChemExpress	10mM/1mL	EUR 135.6

N-Acetyl-D-leucine
GE3308-1G	Glentham Life Sciences	1 g	EUR 88.8

Because of the instability of the genome and clonal evolution is the characteristic of developing myeloid leukemia (CML) (CML), we have relatively examined the separator’s proteolytic activity in the chronic phase cml treated by TKI. Proteolytic separator activity was analyzed at a single cell level in 88 clinical samples and in 14 healthy controls with flow of flow cytometric. In parallel, the expression of the BCR-ABL1 gene and the speed of the replication fork is measured by each with QRT-PCR and DNA fiber, respectively. The distribution value of separator activities (SAD) shows the occurrence of MNC with increased proteolytic activity in the sample found positively correlated with the level of expression of the BCR-ABL1 gene and loss of MMR (relapse) in all routine monitoring of BCR-ABL1.