Separase

A cysteine protease responsible for triggering anaphase by hydrolysing cohesin

Clonal Evolution and Blast Crisis Correlate with Enhanced Proteolytic Activity of Separase in BCR-ABL b3a2 Fusion Type CML under Imatinib Therapy.

Clonal Evolution and Blast Crisis Correlate with Enhanced Proteolytic Activity of Separase in BCR-ABL b3a2 Fusion Type CML under Imatinib Therapy.

Unbalanced (major route) additional cytogenetic aberrations (ACA) in the diagnosis of chronic myeloid leukemia (CML) showed an increase in the risk of progression and shorter survival. In addition, the emerging ACA under imatinib treatment and clonal evolution regarded acceleration features and determine treatment failure in accordance with the European LeukemiaNet (ELN) recommendation.

On the basis of 1151 patients with chronic phase Philadelphia chromosome positive CML random-IV study, we examine recent events, ACA arising under imatinib treatment in connection with p210BCR-ABL breakpoint variants b2a2 and b3a2. We found the ACA preferential acquisition is not balanced in patients with vs b2a2 b3a2 fusion type (ratio: 6.3 vs. 1.6, p = 0.0246) concurring quickly advanced to the blast crisis for b3a2 patients (p = 0.0124).

ESPL1 / Separase, a cysteine ​​endopeptidase, is a key player in chromosome segregation during mitosis. Separase excess and / or hyperactivity have been reported from a variety of cancers and causes defective mitotic spindle, the chromosomes missegregation and aneuploidy. We investigated the influence of p210BCR-ABL breakpoint variants and treatment of imatinib on expression and proteolytic activity of Separase measured by specific fluorogenic assay in CML cell lines (b2a2: KCL-22, BV-173; b3a2: K562, LAMA-84). Despite the decline in protein content Separase up to 5.4-fold increase from Separase activity under imatinib treatment was observed exclusively in b3a2 but not in cell lines b2a2.

Mimic the effect of imatinib on BV-173 and LAMA-84 cells with silencing ESPL1 Separase stimulated proteolytic activity in cell lines of both b3a2 and b2a2. Our data indicate the presence of a feedback mechanism-related types of post- translational fusion Separase stimulates proteolytic activity after therapy-induced decrease in protein levels Separase. This can make b3a2 CML cells more susceptible to aneuploidy and clonal evolution from a common ancestor b2a2 and therefore can explain the results of cytogenetic patients with CML.

Clonal Evolution and Blast Crisis Correlate with Enhanced Proteolytic Activity of Separase in BCR-ABL b3a2 Fusion Type CML under Imatinib Therapy.
Clonal Evolution and Blast Crisis Correlate with Enhanced Proteolytic Activity of Separase in BCR-ABL b3a2 Fusion Type CML under Imatinib Therapy.

Separase Cleaves N-Tail of CENP-A CPAR Related Protein-1 in Meiosis I Metaphase-Anaphase transition in C. elegans.

Epigenetically defined centromere in most eukaryotes by the presence of centromeric chromatin containing histone H3 variant CENP-A. Most species have a single gene that encodes a centromeric histone variant while C. elegans has two: HCP-3 (also known as CeCENP-A) and CPAR-1. Before the replacement RNAi experiments showed that the HCP-3 is a functional isoform dominant, consistent with CPAR-1 does not become detectable in the embryo.

GFP :: CPAR-1 loaded onto meiotic chromosomes in diakinesis and enriched bivalents until meiosis I. Here we show that the CPAR-1 :: GFP signal loss of chromosome segregation precisely coincide with the homologous during anaphase I. The loss of GFP:: CPAR-1 signal reflects the proteolytic cleavage between GFP and histone fold of CPAR-1, as CPAR-1 :: GFP, where GFP fused to the C-terminus of CPAR-1, showed no GFP signal loss.

A focus of the candidates involved separase screen, proteases which initiate anaphase by splitting kleisin subunit of cohesin, in reaction to this division. Examination of the N-terminal tail sequence CPAR-1 expressing separase cleavage site and a mutation of the residues alleged signatures on this site removed cleavage reaction, as visualized by the retention of CPAR-1 :: GFP signal in separating homologous chromosomes in metaphase-anaphase transition of meiosis I.

Separase (phospho Ser801) Rabbit pAb

E2252078 100ul
EUR 225
Description: Available in various conjugation types.

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ES1518-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (phospho Ser801) Polyclonal Antibody

ES1518-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (phospho Ser801) Polyclonal Antibody

E20-60872 100ug
EUR 225
Description: Available in various conjugation types.

Phospho-SEPARASE (Ser801) Cell-Based ELISA Kit

EKA51034-192T 192T
EUR 564.5

Phospho-SEPARASE (Ser801) Cell-Based ELISA Kit

EKA51034-5x96T 5x96T Ask for price

Phospho-SEPARASE (Ser801) Colorimetric Cell-Based ELISA Kit (OKAG01525)

OKAG01525 2 x 96 Wells
EUR 888
Description: Description of target: ;Species reactivity: Human: S801, Mouse: S1189;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: Phospho
Detection Method: Colorimetric 450 nm;Sensitivity:

SEPARASE (Phospho-Ser801) Antibody

E11-0086A 100μg
EUR 225
Description: Available in various conjugation types.

SEPARASE (Phospho-Ser801) Antibody

8A0086 50ug
EUR 368
Description: SEPARASE (Phospho-Ser801) Antibody

Separase (Phospho-Ser801) Polyclonal Antibody

12353-100ul 100ul
EUR 302.4

Separase (Phospho-Ser801) Polyclonal Antibody

12353-50ul 50ul
EUR 224.4

Separase (Phospho-Ser801) Polyclonal Antibody

E012353 100μg/100μl
EUR 255
Description: Available in various conjugation types.

Separase (Phospho-Ser801) Polyclonal Antibody

12353 100ul
EUR 319

Separin Phospho-Ser801 (ESPL1 pS801) Antibody

20-abx326180
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

OASG06575-100UL - ESPL1 Antibody (Phospho-Ser801)

OASG06575-100UL 100ul
EUR 239

OAAF00057-100UG - ESPL1 Antibody (Phospho-Ser801)

OAAF00057-100UG 100ug
EUR 329

Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody

DF8743 200ul
EUR 420

Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody

AF3810-100ul 100ul
EUR 350

Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody

AF3810-200ul 200ul
EUR 450

Phospho-MEK1/2 (Ser218+Ser222/Ser222+Ser226) Antibody

AF8035-100ul 100ul
EUR 350

Phospho-MEK1/2 (Ser218+Ser222/Ser222+Ser226) Antibody

AF8035-200ul 200ul
EUR 450

Phospho-MEK1/2 (Ser218+Ser222/Ser222+Ser226) Antibody

AF8035 100ul
EUR 350
Description: Human,Mouse,Rat

Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody

AF3810 100ul
EUR 350
Description: Human,Mouse,Rat,Monkey

Phospho-STAT5 (Ser725/Ser730)[Ser726/Ser731] Antibody

AF3304-100ul 100ul
EUR 280

Phospho-STAT5 (Ser725/Ser730)[Ser726/Ser731] Antibody

AF3304-200ul 200ul
EUR 350

Phospho-STAT5 (Ser725/Ser730)[Ser726/Ser731] Antibody

AF3304 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-PAK1 /PAK2 (Ser199/Ser204, Ser192/Ser197) Antibody

AF2380-100ul 100ul
EUR 280

Phospho-PAK1 /PAK2 (Ser199/Ser204, Ser192/Ser197) Antibody

AF2380-200ul 200ul
EUR 350

Phospho-PAK1 /PAK2 (Ser199/Ser204, Ser192/Ser197) Antibody

AF2380 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Rabbit mAb

E2R25268 100ul
EUR 255
Description: Available in various conjugation types.

Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) Rabbit mAb

E2R27472 100ul
EUR 255
Description: Available in various conjugation types.

Phospho-PAK1/2/3 (Ser144+Ser141+Ser139) Antibody

DF2948 200ul
EUR 420

Phospho-TAOK1/2/3 (Ser181/Ser181/Ser177) Rabbit mAb

E2R26366 100ul
EUR 255
Description: Available in various conjugation types.

Phospho-PAK1/2/3 (Ser144+Ser141+Ser139) Blocking Peptide

DF2948-BP 1mg
EUR 234

Phospho-CD45 (Ser1007)[Ser1009] Antibody

AF3127-100ul 100ul
EUR 280

Phospho-CD45 (Ser1007)[Ser1009] Antibody

AF3127-200ul 200ul
EUR 350

Phospho-CD45 (Ser1007)[Ser1009] Antibody

AF3127 100ul
EUR 280
Description: Human,Mouse,Rat

PKC mu antibody (Ser738+Ser742)

70R-31523 100 ug
EUR 392.4
Description: Rabbit polyclonal PKC mu antibody (Ser738+Ser742)

MDM2 antibody (Ser186+Ser188)

70R-36311 100 ug
EUR 392.4
Description: Rabbit polyclonal MDM2 antibody (Ser186+Ser188)

PAK5/6 antibody (Ser602/Ser560)

70R-34560 100 ug
EUR 392.4
Description: Rabbit polyclonal PAK5/6 antibody (Ser602/Ser560)

rpS6 antibody (Ser235+Ser236)

70R-34574 100 ug
EUR 392.4
Description: Rabbit polyclonal rpS6 antibody (Ser235+Ser236)

AMPK antibody (Ser485/Ser491)

70R-35208 100 ug
EUR 392.4
Description: Purified Rabbit polyclonal AMPK antibody (Ser485/Ser491)

Phospho-Filamin A/B (Ser2152/Ser2107) Rabbit mAb

E2R26297 100ul
EUR 255
Description: Available in various conjugation types.

Phospho-Filamin A/B (Ser2152/Ser2107) Rabbit mAb

52694 100ul
EUR 499

Phospho- PAK1 (Ser199/204) /PAK2 (Ser192/197) Antibody

ABF3691 100 ug
EUR 525.6

ADRB2 antibody (Ser355+Ser356)

70R-33353 100 ug
EUR 392.4
Description: Rabbit polyclonal ADRB2 antibody (Ser355+Ser356)

AMPK1 antibody (Ser485/Ser491)

70R-37249 100 ug
EUR 418.8
Description: Rabbit Polyclonal AMPK1 antibody (Ser485/Ser491)

NFAT3 antibody (Ser168+Ser170)

70R-34549 100 ug
EUR 392.4
Description: Rabbit polyclonal NFAT3 antibody (Ser168+Ser170)

PPP1R2 antibody (Ser120+Ser121)

70R-36672 100 ug
EUR 392.4
Description: Rabbit Polyclonal PPP1R2 antibody (Ser120+Ser121)

FOXO1A antibody (Ser322+Ser325)

70R-35267 100 ug
EUR 392.4
Description: Purified Rabbit polyclonal FOXO1A antibody (Ser322+Ser325)

Phospho-Beclin 1 (Ser90/Ser93/Ser96) Antibody

AF7386-100ul 100ul
EUR 350

Phospho-Beclin 1 (Ser90/Ser93/Ser96) Antibody

AF7386-200ul 200ul
EUR 450

Phospho-Beclin 1 (Ser90/Ser93/Ser96) Antibody

AF7386 100ul
EUR 350
Description: Human,Mouse,Rat

Phospho- Sox2 (Ser250/Ser251) Antibody

ABF3753 100 ug
EUR 525.6

MEK1/2 (Phospho- Ser217+ Ser221) Antibody

ABF8035 100 ug
EUR 525.6

EIF5 (Phospho- Ser389+ Ser390) Antibody

ABF8473 100 ug
EUR 525.6

MYPT1/2 (Phospho Ser668/Ser618) rabbit pAb

E28PP1612 100μl
EUR 255
Description: Available in various conjugation types.

Phospho-AR (Ser650)[Ser651] Antibody

AF3137-100ul 100ul
EUR 280

Phospho-AR (Ser650)[Ser651] Antibody

AF3137-200ul 200ul
EUR 350

Phospho-AR (Ser650)[Ser651] Antibody

AF3137 100ul
EUR 280
Description: Human

Smad1/5/9 (Phospho- Ser463+ Ser465) Antibody

ABF8313 100 ug
EUR 525.6

Smad2 (Phospho- Ser465+ Ser467) Antibody

ABF8314 100 ug
EUR 525.6

Smad3 (Phospho- Ser423+ Ser425) Antibody

ABF8315 100 ug
EUR 525.6

MYPT1/2 (Phospho- Ser668/Ser618) Antibody

ABF8447 100 ug
EUR 525.6

Smad1/9 (Phospho- Ser463/Ser465) Antibody

ABF8454 100 ug
EUR 525.6

Phospho-HSF1 (Ser303/Ser307) Rabbit mAb

E2R26307 100ul
EUR 255
Description: Available in various conjugation types.

Phospho-HSL (Ser563)[Ser853] Antibody

AF2350-100ul 100ul
EUR 280

Phospho-HSL (Ser563)[Ser853] Antibody

AF2350-200ul 200ul
EUR 350

Phospho-MFF (Ser172/Ser146) Antibody

AF2365-100ul 100ul
EUR 280

Phospho-MFF (Ser172/Ser146) Antibody

AF2365-200ul 200ul
EUR 350

Phospho-p63 (Ser160/Ser162) Antibody

AF2379-100ul 100ul
EUR 280

Phospho-p63 (Ser160/Ser162) Antibody

AF2379-200ul 200ul
EUR 350

Phospho-Tau (Ser199)[Ser516] Antibody

AF2418-100ul 100ul
EUR 280

Phospho-Tau (Ser199)[Ser516] Antibody

AF2418-200ul 200ul
EUR 350

Phospho-Tau (Ser202)[Ser519] Antibody

AF2419-100ul 100ul
EUR 280

Phospho-Tau (Ser202)[Ser519] Antibody

AF2419-200ul 200ul
EUR 350

Phospho-Tau (Ser416)[Ser733] Antibody

AF2420-100ul 100ul
EUR 280

Phospho-Tau (Ser416)[Ser733] Antibody

AF2420-200ul 200ul
EUR 350

Phospho-Tau (Ser356)[Ser673] Antibody

AF3143-100ul 100ul
EUR 280

Phospho-Tau (Ser356)[Ser673] Antibody

AF3143-200ul 200ul
EUR 350

Phospho-Tau (Ser404)[Ser721] Antibody

AF3144-100ul 100ul
EUR 280

Phospho-Tau (Ser404)[Ser721] Antibody

AF3144-200ul 200ul
EUR 350

Phospho-Tau (Ser422)[Ser739] Antibody

AF3145-100ul 100ul
EUR 280

Phospho-Tau (Ser422)[Ser739] Antibody

AF3145-200ul 200ul
EUR 350

Phospho-Tau (Ser262)[Ser579] Antibody

AF3151-100ul 100ul
EUR 280

Phospho-Tau (Ser262)[Ser579] Antibody

AF3151-200ul 200ul
EUR 350

Phospho-HSL (Ser855/Ser554) Antibody

AF3402-100ul 100ul
EUR 280

Phospho-HSL (Ser855/Ser554) Antibody

AF3402-200ul 200ul
EUR 350

Phospho-BAD (Ser155)[Ser118] Antibody

AF3471-100ul 100ul
EUR 280

Phospho-BAD (Ser155)[Ser118] Antibody

AF3471-200ul 200ul
EUR 350

Phospho-Tau (Ser202/Ser519) Antibody

AF3947-100ul 100ul
EUR 350

Phospho-Tau (Ser202/Ser519) Antibody

AF3947-200ul 200ul
EUR 450

Phospho-Rel (Ser492/Ser460) Antibody

AF8126-100ul 100ul
EUR 350

Phospho-Rel (Ser492/Ser460) Antibody

AF8126-200ul 200ul
EUR 450

Phospho-Tau (Ser717/Ser400) Antibody

AF8129-100ul 100ul
EUR 350

Phospho-Tau (Ser717/Ser400) Antibody

AF8129-200ul 200ul
EUR 450

Phospho-Rel (Ser492/Ser460) Antibody

AF8126 100ul
EUR 350
Description: Human,Mouse,Rat

Phospho-Tau (Ser717/Ser400) Antibody

AF8129 100ul
EUR 350
Description: Human,Mouse,Rat

Phospho-HSL (Ser855/Ser554) Antibody

AF3402 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-BAD (Ser155)[Ser118] Antibody

AF3471 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-Tau (Ser202/Ser519) Antibody

AF3947 100ul
EUR 350
Description: Human,Mouse,Rat

Phospho-HSL (Ser563)[Ser853] Antibody

AF2350 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-MFF (Ser172/Ser146) Antibody

AF2365 100ul
EUR 280
Description: Human

Phospho-p63 (Ser160/Ser162) Antibody

AF2379 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-Tau (Ser199)[Ser516] Antibody

AF2418 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-Tau (Ser202)[Ser519] Antibody

AF2419 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-Tau (Ser416)[Ser733] Antibody

AF2420 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-Tau (Ser356)[Ser673] Antibody

AF3143 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-Tau (Ser404)[Ser721] Antibody

AF3144 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-Tau (Ser422)[Ser739] Antibody

AF3145 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-Tau (Ser262)[Ser579] Antibody

AF3151 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Blocking Peptide

AF2380-BP 1mg
EUR 234

Phospho-MDM2 (Ser186/Ser188) Antibody

CSB-PA570037- each
EUR 402
Description: A polyclonal antibody against Phospho-MDM2 (Ser186/Ser188). Recognizes Phospho-MDM2 (Ser186/Ser188) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:200

Phospho-MDM2 (Ser186/Ser188) Antibody

CSB-PA570037-100ul 100ul
EUR 434.4
Description: A polyclonal antibody against Phospho-MDM2 (Ser186/Ser188). Recognizes Phospho-MDM2 (Ser186/Ser188) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:200

Phospho-Smad1 (Ser463/Ser465) Rabbit mAb

E2R26360 100ul
EUR 255
Description: Available in various conjugation types.

Phospho-KSR1 (Ser392)[Ser460] Antibody

AF2358-100ul 100ul
EUR 280

Phospho-KSR1 (Ser392)[Ser460] Antibody

AF2358-200ul 200ul
EUR 350

Phospho-MKP1/2 (Ser296/Ser318) Antibody

AF3243-100ul 100ul
EUR 280

Phospho-MKP1/2 (Ser296/Ser318) Antibody

AF3243-200ul 200ul
EUR 350

Phospho-MEK1/2 (Ser217)[Ser218] Antibody

AF3384-100ul 100ul
EUR 280

Phospho-MEK1/2 (Ser217)[Ser218] Antibody

AF3384-200ul 200ul
EUR 350

Phospho-MEK1/2 (Ser221)[Ser222] Antibody

AF3385-100ul 100ul
EUR 280

Phospho-MEK1/2 (Ser221)[Ser222] Antibody

AF3385-200ul 200ul
EUR 350

Phospho-PAK5/6 (Ser602/Ser560) Antibody

AF3522-100ul 100ul
EUR 350

Phospho-PAK5/6 (Ser602/Ser560) Antibody

AF3522-200ul 200ul
EUR 450

Phospho-GBF1 (Ser292/Ser297) Antibody

AF3565-100ul 100ul
EUR 350

Phospho-GBF1 (Ser292/Ser297) Antibody

AF3565-200ul 200ul
EUR 450

Phospho-n-Myc (Ser261/Ser263) Antibody

AF3750-100ul 100ul
EUR 350

Neither whether or uncleavable CPAR-1 centromere-localized in mitosis and not locally around chromatin, indicating that activity has not been retained in the CPAR centromere-1. Although the function of CPAR-1 and cleavage depends separase that remains to be explained, these efforts reveal new substrate separase and provide in vivo biosensors to monitor activity at the beginning of meiosis I separase anaphase.

February 2024
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