Structure and Function of the Separase-Securin Complex

Structure and Function of the Separase-Securin Complex

Sepaase is a large protease of cysteine ​​in eukaryotes and presents crucial roles in many cell processes, in particular chromosomal segregation during mitosis and meiosis, apoptosis, damage repair of DNA, from the disengagement of the centrosome and duplication, stabilization and elongation of the spindle. It dissolves the cohesion between sister chromatids by cleaving one of the subheadings of the cohesin ring for chromosome segregation. The sepaase activity is tightly controlled at several levels, by the direct binding of inhibitory proteins as well as the post-procedure modification. The dysregulation of SEARAISE business is related to cancer and genome instability, making it a drug discovery target. One of the most famous sepaase inhibitors is securin, which has been identified in yeast, plants and animals.

Securin forms a tight complex with sepaase and inhibits porceously its catalytic activity. The recent structures of the Sepase-Securin complex revealed the molecular mechanism of the safety inhibitor activity. A segment of securin is linked in the active sepaase site, thus blocking the substrate bonding. Securin itself is not cleaved by Separase because its link mode is not compatible with catalysis. Securin also has extensive interactions with sepaase outside the active site, according to its chaperone function to stabilize this enzyme. In several species, including Xenopus, the mouse and the man, two members of the Cyclin family have been identified. Cyclin A2, which is expressed ubiquitously in dividing cells and plays a role in the replication of DNA, entry into mitosis and the spindle assembly and cyclin A1, whose function is less clear and which is expressed in spermatocytes, leukemia cells and postmicathic multicile cells.

The suppression of the gene has shown that cyclin A1 is essential for masculine meiosis, but not essential for female meiosis. Our results revealed that the cyclin A1 is not only dispensable in the oocytes, we show here that its expression is actually undesirable in these cells. Our data demonstrate that CPA / C and oocyte proteasome are unable to target the cyclin A1 sufficiently prior to anchhase, which causes an arrest of anaphase and a direct inhibition of separation.

 

 

Separation of chromosomes during the masculine meiosis of Drosophila, I need a separate-mediated cleavage of the Uno homologous conjunction protein

 

Regular chromosomal segregation during the first meotic division requires a preliminary matching of homologous chromosomes in bivalent. During canonical meiosis, the bond between homologous chromosomes is maintained until the end of the metaphase I by chiasmata resulting from metiotic recombination in combination with a distal sister chromatide cohesion. Sepased removal of the cohesin of the chromosome arms at the end of the metaphase I allows the resolution of the chiasmata and the homologation to opposite spindle poles during an anaphase I. is interesting, sepaase is also required for The bivalent division during meiosis I in the males of Drosophila, where counterparts are jointly by an alternative mechanism independent of meiotic recombination and cohesin.

Here, we indicate the identification of a new alternative homologous conjunction protein encoded by previously increed genes uniforms only (UNO). Univalent in non-zero mutants at the beginning of meiosis I, instead of normal bivalents, are randomly separated. In the wild type, the UNO protein is detected in points associated with bivalent chromosomes and most abundantly at the localized twinning site of sexual chromosomes. Uno is cleaved by sepaase.

The expression of a single-mutant version with a non-functional sepaase cleavage site restores the counterpart of the homologous conjunction in a bottom of the UNO null. However, the separation of bivalent during meiosis, I am completely repealed by this unravelful version of the United Nations. Therefore, we propose that the homologous separation during the male meiosis of Drosophila is triggered by a separately-mediated divide of the UNO.

 Circulating dipeptidyl peptidase-4 is independently associated with the presence and severity of NAFLD/NASH in individuals with and without obesity and metabolic disease
Circulating dipeptidyl peptidase-4 is independently associated with the presence and severity of NAFLD/NASH in individuals with and without obesity and metabolic disease

Centrosometive reduction of newly generated tetraploid cancer cells obtained by depletion of separation

 

Tetraploidy, a common characteristic of cancer, leads to the presence of additional centrosomes, associated with chromosomal instability (CIN) and aneuploidy. Deregulation in the number of centricsomes triggers tumorigenesis. However, how to evolve supernumerary centrics when the emergence of tetraploid cells remains elucidated. Here, generating tetraploid isogenic clones in colorectal cancer and in unprocessed cells, we show that almost tetraploid clones have a significant increase in the number of centricsomes. In addition, we find that the centrous region in quasi-tetraploids is twice as big as in the almost diploids. To assess whether the centrosome clustering would occur, we then analyzed the number of hundreds revealing the amplification of the hundred. Nevertheless, more than half of the quasi-tetraploids maintained in the culture do not have centrosomal aberrations. To determine if the cells have gradually lost the hundreds after becoming near tetraploids, we transfer transfected diploid cells with SIRNA against espl1 / separase, a protease responsible for triggering an anaphase, in order to generate newly tetraploid cells.

Finally, using this model, we evaluated the number of hundreds at different points of time after the tetrapolisation of the search for quasi-tetraploid rapidly losing centrosomes over time. Taken together, these data demonstrate that, although most cells reduce supernumerary centrosomes after tetrapolisation, a small fraction retains additional cottages, which potentially leads to CIN. The arrest of cyclin-induced cell cycle A1 is specific to the oocytes and the presence of cyclin A1 in early embryos has no effect on the progression of the cell cycle or the chromosomal division.

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Separase antibody

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Description: Purified Polyclonal Separase antibody

SEPARASE antibody

70R-31054 100 ug
EUR 392.4
Description: Rabbit polyclonal SEPARASE antibody

Separase Antibody

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Separase Antibody

44415-50ul 50ul
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Separase Antibody

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  • 100 ul
  • 200 ul
  • 30 ul

Separase Antibody

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Separase Antibody

20-abx215242
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

anti-Separase

YF-PA25396 50 ul
EUR 400.8
Description: Mouse polyclonal to Separase

Separase Polyclonal Antibody

ABP52424-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ABP52424-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ABP52424-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

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Separase Polyclonal Antibody

ES3423-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase Polyclonal Antibody

ES3423-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

SEPARASE antibody (Ser801)

70R-31053 100 ug
EUR 392.4
Description: Rabbit polyclonal SEPARASE antibody (Ser801)

Separase (pS801) Antibody

20-abx134111
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  • EUR 560.40
  • EUR 243.60
  • 100 ul
  • 200 ul
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Anti-Separase Antibody

A04364 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Separase Antibody (ESPL1) detection. Tested with WB, IHC, IF in Human, Mouse.

Anti-Separase antibody

STJ95608 200 µl
EUR 236.4
Description: Rabbit polyclonal to Separase.

Anti-Separase (6H6)

YF-MA11199 100 ug
EUR 435.6
Description: Mouse monoclonal to Separase

SEPARASE Cell ELISA Kit

abx595539-96tests 96 tests
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C-Peptide Blocking Peptide

3277BP-50 each
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Separase (phospho Ser801) Polyclonal Antibody

ABP50519-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ES1518-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (phospho Ser801) Polyclonal Antibody

ES1518-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (Phospho-Ser801) Polyclonal Antibody

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Separase (Phospho-Ser801) Polyclonal Antibody

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Anti-Phospho-Separase (S801) antibody

STJ91032 200 µl
EUR 236.4
Description: Rabbit polyclonal to Phospho-Separase (S801).

UBE2E3 Blocking Peptide

DF10000-BP 1mg
EUR 234

UBE2G1 Blocking Peptide

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EUR 234

UBE2U Blocking Peptide

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KIAA0087 Blocking Peptide

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EUR 234

ATP6V1B2 Blocking Peptide

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EUR 234

ATP6V1G3 Blocking Peptide

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EUR 234

ATP6V0A2 Blocking Peptide

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VCAN Blocking Peptide

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EUR 234

GOLT1A Blocking Peptide

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EUR 234

SFT2D3 Blocking Peptide

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SEC22B Blocking Peptide

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TMEM37 Blocking Peptide

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CACNA1D Blocking Peptide

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CACNA1E Blocking Peptide

DF10013-BP 1mg
EUR 234

CACNA1G Blocking Peptide

DF10014-BP 1mg
EUR 234

ZNHIT1 Blocking Peptide

DF10015-BP 1mg
EUR 234

ZFHX4 Blocking Peptide

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EUR 234

ZNF295 Blocking Peptide

DF10017-BP 1mg
EUR 234

ZNF462 Blocking Peptide

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EUR 234

ZNF600 Blocking Peptide

DF10019-BP 1mg
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SLC30A2 Blocking Peptide

DF10020-BP 1mg
EUR 234

SLC39A10 Blocking Peptide

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ZP2 Blocking Peptide

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EUR 234

CD164L2 Blocking Peptide

DF10023-BP 1mg
EUR 234

CC50A Blocking Peptide

DF10024-BP 1mg
EUR 234

CCNI Blocking Peptide

DF10025-BP 1mg
EUR 234

CD2L5 Blocking Peptide

DF10026-BP 1mg
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CD2L6 Blocking Peptide

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EUR 234

CD2L7 Blocking Peptide

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MAGA2 Blocking Peptide

DF10029-BP 1mg
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MAGA3 Blocking Peptide

DF10030-BP 1mg
EUR 234

MAGA6 Blocking Peptide

DF10031-BP 1mg
EUR 234

MAGA8 Blocking Peptide

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MAGAB Blocking Peptide

DF10033-BP 1mg
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MAGAC Blocking Peptide

DF10034-BP 1mg
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MAGB2 Blocking Peptide

DF10035-BP 1mg
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MAGB3 Blocking Peptide

DF10036-BP 1mg
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MAGB6 Blocking Peptide

DF10037-BP 1mg
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MAGBA Blocking Peptide

DF10038-BP 1mg
EUR 234

MAGBI Blocking Peptide

DF10039-BP 1mg
EUR 234

MAGD2 Blocking Peptide

DF10040-BP 1mg
EUR 234

MAGE2 Blocking Peptide

DF10041-BP 1mg
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MAGG1 Blocking Peptide

DF10042-BP 1mg
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MAGH1 Blocking Peptide

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KITH_HHV1E Blocking Peptide

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CNBP Blocking Peptide

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EUR 234

HEN2 Blocking Peptide

DF10046-BP 1mg
EUR 234

ATOH8 Blocking Peptide

DF10047-BP 1mg
EUR 234

AKAP4 Blocking Peptide

DF10048-BP 1mg
EUR 234

GFI1B Blocking Peptide

DF10049-BP 1mg
EUR 234

DMRTA1 Blocking Peptide

DF10050-BP 1mg
EUR 234

ZNF25 Blocking Peptide

DF10051-BP 1mg
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TSH3 Blocking Peptide

DF10052-BP 1mg
EUR 234

LARP2 Blocking Peptide

DF10053-BP 1mg
EUR 234

UL97 Blocking Peptide

DF10054-BP 1mg
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TRIM68 Blocking Peptide

DF10055-BP 1mg
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TCF2 Blocking Peptide

DF10056-BP 1mg
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ZNF839 Blocking Peptide

DF10057-BP 1mg
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PTRF Blocking Peptide

DF10058-BP 1mg
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CYFIP1 Blocking Peptide

DF10059-BP 1mg
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TRMT11 Blocking Peptide

DF10060-BP 1mg
EUR 234

ZNF227 Blocking Peptide

DF10061-BP 1mg
EUR 234

DMRTC2 Blocking Peptide

DF10062-BP 1mg
EUR 234

YAF2 Blocking Peptide

DF10063-BP 1mg
EUR 234

ASPM Blocking Peptide

DF10064-BP 1mg
EUR 234

ZNF786 Blocking Peptide

DF10065-BP 1mg
EUR 234

C16orf44 Blocking Peptide

DF10066-BP 1mg
EUR 234

LZTR1 Blocking Peptide

DF10067-BP 1mg
EUR 234

ZNF441 Blocking Peptide

DF10068-BP 1mg
EUR 234

TUSC4 Blocking Peptide

DF10069-BP 1mg
EUR 234

TP73 Blocking Peptide

DF10070-BP 1mg
EUR 234

MINA Blocking Peptide

DF10071-BP 1mg
EUR 234

CI098 Blocking Peptide

DF10072-BP 1mg
EUR 234

DUX5 Blocking Peptide

DF10073-BP 1mg
EUR 234

CIC Blocking Peptide

DF10074-BP 1mg
EUR 234

NEB2 Blocking Peptide

DF10075-BP 1mg
EUR 234

MYCBP Blocking Peptide

DF10076-BP 1mg
EUR 234

ZNF577 Blocking Peptide

DF10078-BP 1mg
EUR 234

HIPK3 Blocking Peptide

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EUR 234

POLM Blocking Peptide

DF10080-BP 1mg
EUR 234

NOLA1 Blocking Peptide

DF10081-BP 1mg
EUR 234

TF2L1 Blocking Peptide

DF10082-BP 1mg
EUR 234

VPS54 Blocking Peptide

DF10083-BP 1mg
EUR 234

RHCG Blocking Peptide

DF10084-BP 1mg
EUR 234

RTDR1 Blocking Peptide

DF10086-BP 1mg
EUR 234

JDP2 Blocking Peptide

DF10087-BP 1mg
EUR 234

CDYL1 Blocking Peptide

DF10088-BP 1mg
EUR 234

DUET Blocking Peptide

DF10089-BP 1mg
EUR 234

CDY1 Blocking Peptide

DF10090-BP 1mg
EUR 234

CCBE1 Blocking Peptide

DF10092-BP 1mg
EUR 234

COX7B Blocking Peptide

DF10093-BP 1mg
EUR 234

COX7A2L Blocking Peptide

DF10094-BP 1mg
EUR 234

COX82 Blocking Peptide

DF10095-BP 1mg
EUR 234

COX6A2 Blocking Peptide

DF10096-BP 1mg
EUR 234

COX6B2 Blocking Peptide

DF10097-BP 1mg
EUR 234

COX7A1 Blocking Peptide

DF10098-BP 1mg
EUR 234

CYBA Blocking Peptide

DF10099-BP 1mg
EUR 234

DEDD Blocking Peptide

DF10100-BP 1mg
EUR 234

DNAJB2 Blocking Peptide

DF10101-BP 1mg
EUR 234

EMID2 Blocking Peptide

DF10102-BP 1mg
EUR 234

FBF1 Blocking Peptide

DF10103-BP 1mg
EUR 234

GRIK5 Blocking Peptide

DF10104-BP 1mg
EUR 234

GRIP2 Blocking Peptide

DF10105-BP 1mg
EUR 234

IL20L2 Blocking Peptide

DF10106-BP 1mg
EUR 234

IL22RA2 Blocking Peptide

DF10107-BP 1mg
EUR 234

IL28RA Blocking Peptide

DF10108-BP 1mg
EUR 234

IFNW1 Blocking Peptide

DF10109-BP 1mg
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IGFL2 Blocking Peptide

DF10110-BP 1mg
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Cyclin A1 is therefore not only a large cellular cycle regulator with germinal-biased expression, essential for men and damaging for female meiosis, persistent expression during anaphase in oocytes shows fundamental differences between APC / C in early ecytes and embryos.

Karina

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