Structure and Function of the Separase-Securin Complex

Structure and Function of the Separase-Securin Complex

Sepaase is a large protease of cysteine ​​in eukaryotes and presents crucial roles in many cell processes, in particular chromosomal segregation during mitosis and meiosis, apoptosis, damage repair of DNA, from the disengagement of the centrosome and duplication, stabilization and elongation of the spindle. It dissolves the cohesion between sister chromatids by cleaving one of the subheadings of the cohesin ring for chromosome segregation. The sepaase activity is tightly controlled at several levels, by the direct binding of inhibitory proteins as well as the post-procedure modification. The dysregulation of SEARAISE business is related to cancer and genome instability, making it a drug discovery target. One of the most famous sepaase inhibitors is securin, which has been identified in yeast, plants and animals.

Securin forms a tight complex with sepaase and inhibits porceously its catalytic activity. The recent structures of the Sepase-Securin complex revealed the molecular mechanism of the safety inhibitor activity. A segment of securin is linked in the active sepaase site, thus blocking the substrate bonding. Securin itself is not cleaved by Separase because its link mode is not compatible with catalysis. Securin also has extensive interactions with sepaase outside the active site, according to its chaperone function to stabilize this enzyme. In several species, including Xenopus, the mouse and the man, two members of the Cyclin family have been identified. Cyclin A2, which is expressed ubiquitously in dividing cells and plays a role in the replication of DNA, entry into mitosis and the spindle assembly and cyclin A1, whose function is less clear and which is expressed in spermatocytes, leukemia cells and postmicathic multicile cells.

The suppression of the gene has shown that cyclin A1 is essential for masculine meiosis, but not essential for female meiosis. Our results revealed that the cyclin A1 is not only dispensable in the oocytes, we show here that its expression is actually undesirable in these cells. Our data demonstrate that CPA / C and oocyte proteasome are unable to target the cyclin A1 sufficiently prior to anchhase, which causes an arrest of anaphase and a direct inhibition of separation.

 

 

Separation of chromosomes during the masculine meiosis of Drosophila, I need a separate-mediated cleavage of the Uno homologous conjunction protein

 

Regular chromosomal segregation during the first meotic division requires a preliminary matching of homologous chromosomes in bivalent. During canonical meiosis, the bond between homologous chromosomes is maintained until the end of the metaphase I by chiasmata resulting from metiotic recombination in combination with a distal sister chromatide cohesion. Sepased removal of the cohesin of the chromosome arms at the end of the metaphase I allows the resolution of the chiasmata and the homologation to opposite spindle poles during an anaphase I. is interesting, sepaase is also required for The bivalent division during meiosis I in the males of Drosophila, where counterparts are jointly by an alternative mechanism independent of meiotic recombination and cohesin.

Here, we indicate the identification of a new alternative homologous conjunction protein encoded by previously increed genes uniforms only (UNO). Univalent in non-zero mutants at the beginning of meiosis I, instead of normal bivalents, are randomly separated. In the wild type, the UNO protein is detected in points associated with bivalent chromosomes and most abundantly at the localized twinning site of sexual chromosomes. Uno is cleaved by sepaase.

The expression of a single-mutant version with a non-functional sepaase cleavage site restores the counterpart of the homologous conjunction in a bottom of the UNO null. However, the separation of bivalent during meiosis, I am completely repealed by this unravelful version of the United Nations. Therefore, we propose that the homologous separation during the male meiosis of Drosophila is triggered by a separately-mediated divide of the UNO.

 Circulating dipeptidyl peptidase-4 is independently associated with the presence and severity of NAFLD/NASH in individuals with and without obesity and metabolic disease
Circulating dipeptidyl peptidase-4 is independently associated with the presence and severity of NAFLD/NASH in individuals with and without obesity and metabolic disease

Centrosometive reduction of newly generated tetraploid cancer cells obtained by depletion of separation

 

Tetraploidy, a common characteristic of cancer, leads to the presence of additional centrosomes, associated with chromosomal instability (CIN) and aneuploidy. Deregulation in the number of centricsomes triggers tumorigenesis. However, how to evolve supernumerary centrics when the emergence of tetraploid cells remains elucidated. Here, generating tetraploid isogenic clones in colorectal cancer and in unprocessed cells, we show that almost tetraploid clones have a significant increase in the number of centricsomes. In addition, we find that the centrous region in quasi-tetraploids is twice as big as in the almost diploids. To assess whether the centrosome clustering would occur, we then analyzed the number of hundreds revealing the amplification of the hundred. Nevertheless, more than half of the quasi-tetraploids maintained in the culture do not have centrosomal aberrations. To determine if the cells have gradually lost the hundreds after becoming near tetraploids, we transfer transfected diploid cells with SIRNA against espl1 / separase, a protease responsible for triggering an anaphase, in order to generate newly tetraploid cells.

Finally, using this model, we evaluated the number of hundreds at different points of time after the tetrapolisation of the search for quasi-tetraploid rapidly losing centrosomes over time. Taken together, these data demonstrate that, although most cells reduce supernumerary centrosomes after tetrapolisation, a small fraction retains additional cottages, which potentially leads to CIN. The arrest of cyclin-induced cell cycle A1 is specific to the oocytes and the presence of cyclin A1 in early embryos has no effect on the progression of the cell cycle or the chromosomal division.

Separase (pS801) Blocking Peptide

20-abx162232
  • EUR 314.00
  • EUR 509.00
  • 1 mg
  • 5 mg

Separase Antibody

20-abx121903
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Separase Antibody

20-abx215242
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Separase Antibody

44415-100ul 100ul
EUR 252

Separase Antibody

44415-50ul 50ul
EUR 187

Separase Antibody

AF9195 200ul
EUR 304
Description: Separase Antibody detects endogenous levels of total Separase.

Separase Antibody

ABF9195 100 ug
EUR 438

anti-Separase

YF-PA25396 50 ul
EUR 334
Description: Mouse polyclonal to Separase

SEPARASE antibody

70R-31054 100 ug
EUR 327
Description: Rabbit polyclonal SEPARASE antibody

Separase antibody

70R-51689 100 ul
EUR 287
Description: Purified Polyclonal Separase antibody

Separase Polyclonal Antibody

ES3423-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase Polyclonal Antibody

ES3423-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Anti-Separase Antibody

A04364 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Separase Antibody (ESPL1) detection. Tested with WB, IHC, IF in Human, Mouse.

Separase Polyclonal Antibody

ABP52424-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ABP52424-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ABP52424-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase (pS801) Antibody

20-abx134111
  • EUR 314.00
  • EUR 467.00
  • EUR 203.00
  • 100 ul
  • 200 ul
  • 30 ul

Anti-Separase antibody

STJ95608 200 µl
EUR 197
Description: Rabbit polyclonal to Separase.

Separase Conjugated Antibody

C44415 100ul
EUR 397

Anti-Separase (6H6)

YF-MA11199 100 ug
EUR 363
Description: Mouse monoclonal to Separase

SEPARASE antibody (Ser801)

70R-31053 100 ug
EUR 327
Description: Rabbit polyclonal SEPARASE antibody (Ser801)

SEPARASE Cell ELISA Kit

abx595539-96tests 96 tests
EUR 637

Separase (phospho Ser801) Polyclonal Antibody

ES1518-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (phospho Ser801) Polyclonal Antibody

ES1518-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (Phospho-Ser801) Polyclonal Antibody

12353-100ul 100ul
EUR 252

Separase (Phospho-Ser801) Polyclonal Antibody

12353-50ul 50ul
EUR 187

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Anti-Phospho-Separase (S801) antibody

STJ91032 200 µl
EUR 197
Description: Rabbit polyclonal to Phospho-Separase (S801).

SEPARASE Colorimetric Cell-Based ELISA Kit

EKC1516 100ul
EUR 572

Polyclonal ESPL1 / Separase Antibody (aa767-816)

APR02989G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ESPL1 / Separase (aa767-816). This antibody is tested and proven to work in the following applications:

C-Peptide Blocking Peptide

3277BP-50
EUR 153

SEPARASE Colorimetric Cell-Based ELISA Kit (OKAG01028)

OKAG01028 96 Wells
EUR 596
Description: Description of target: ;Species reactivity: Human, Mouse;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:

Canine C-Peptide ELISA Kit

RD-C-Peptide-c-48Tests 48 Tests
EUR 533

Canine C-Peptide ELISA Kit

RD-C-Peptide-c-96Tests 96 Tests
EUR 740

Human C-Peptide ELISA Kit

RD-C-Peptide-Hu-48Tests 48 Tests
EUR 387

Human C-Peptide ELISA Kit

RD-C-Peptide-Hu-96Tests 96 Tests
EUR 532

Mouse C-Peptide ELISA Kit

RD-C-Peptide-Mu-48Tests 48 Tests
EUR 446

Mouse C-Peptide ELISA Kit

RD-C-Peptide-Mu-96Tests 96 Tests
EUR 615

Rat C-Peptide ELISA Kit

RD-C-Peptide-Ra-48Tests 48 Tests
EUR 465

Rat C-Peptide ELISA Kit

RD-C-Peptide-Ra-96Tests 96 Tests
EUR 643

Canine C-Peptide ELISA Kit

DLR-C-Peptide-c-48T 48T
EUR 527
Description: A competitive inhibition quantitative ELISA assay kit for detection of Canine C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine C-Peptide ELISA Kit

DLR-C-Peptide-c-96T 96T
EUR 688
Description: A competitive inhibition quantitative ELISA assay kit for detection of Canine C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human C-Peptide ELISA Kit

DLR-C-Peptide-Hu-48T 48T
EUR 398
Description: A competitive inhibition quantitative ELISA assay kit for detection of Human C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human C-Peptide ELISA Kit

DLR-C-Peptide-Hu-96T 96T
EUR 511
Description: A competitive inhibition quantitative ELISA assay kit for detection of Human C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse C-Peptide ELISA Kit

DLR-C-Peptide-Mu-48T 48T
EUR 450
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse C-Peptide ELISA Kit

DLR-C-Peptide-Mu-96T 96T
EUR 582
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat C-Peptide ELISA Kit

DLR-C-Peptide-Ra-48T 48T
EUR 467
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat C-Peptide ELISA Kit

DLR-C-Peptide-Ra-96T 96T
EUR 605
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine C-Peptide ELISA Kit

RDR-C-Peptide-c-48Tests 48 Tests
EUR 557

Canine C-Peptide ELISA Kit

RDR-C-Peptide-c-96Tests 96 Tests
EUR 774

Human C-Peptide ELISA Kit

RDR-C-Peptide-Hu-48Tests 48 Tests
EUR 404

Human C-Peptide ELISA Kit

RDR-C-Peptide-Hu-96Tests 96 Tests
EUR 556

Mouse C-Peptide ELISA Kit

RDR-C-Peptide-Mu-48Tests 48 Tests
EUR 465

Mouse C-Peptide ELISA Kit

RDR-C-Peptide-Mu-96Tests 96 Tests
EUR 643

Rat C-Peptide ELISA Kit

RDR-C-Peptide-Ra-48Tests 48 Tests
EUR 486

Rat C-Peptide ELISA Kit

RDR-C-Peptide-Ra-96Tests 96 Tests
EUR 672

CD39 Blocking Peptide

P10103 20 ug Blocking Peptide
EUR 146

P2X3 Blocking Peptide

P10108 100 ug Blocking Peptide
EUR 146

CXCL1 Blocking Peptide

20-abx061039
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

p21 Blocking Peptide

20-abx061043
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

Dyskerin Blocking Peptide

20-abx061050
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

ZNF265 Blocking Peptide

20-abx061051
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

CDK11B Blocking Peptide

20-abx061053
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

HMGB2 Blocking Peptide

20-abx061056
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

ZFP36L2 Blocking Peptide

20-abx061059
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

CBP20 Blocking Peptide

20-abx061060
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

FOXE3 Blocking Peptide

20-abx061063
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

CBFB Blocking Peptide

20-abx061064
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

MAPKAPK3 Blocking Peptide

20-abx061065
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

MOB3C Blocking Peptide

20-abx061067
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

DPF2 Blocking Peptide

20-abx061068
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

TAF15 Blocking Peptide

20-abx061069
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

FAM84B Blocking Peptide

20-abx061070
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

GTF2IRD1 Blocking Peptide

20-abx061071
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

COL19A1 Blocking Peptide

20-abx061072
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

CYP2W1 Blocking Peptide

20-abx061073
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

RAD21 Blocking Peptide

20-abx061075
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

PSMC3 Blocking Peptide

20-abx061076
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

PSMD11 Blocking Peptide

20-abx061077
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

MRPS9 Blocking Peptide

20-abx061078
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

MRPL41 Blocking Peptide

20-abx061079
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

MRPL48 Blocking Peptide

20-abx061080
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

RPS12 Blocking Peptide

20-abx061081
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

RPS13 Blocking Peptide

20-abx061082
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

ACSS1 Blocking Peptide

20-abx061084
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

AKR1C2 Blocking Peptide

20-abx061085
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

ACER3 Blocking Peptide

20-abx061086
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

ATP5I Blocking Peptide

20-abx061087
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

ATP5C1 Blocking Peptide

20-abx061088
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

CNTROB Blocking Peptide

20-abx061089
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

CEP78 Blocking Peptide

20-abx061090
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

DMGDH Blocking Peptide

20-abx061093
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

EIF3D Blocking Peptide

20-abx061095
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

FUBP3 Blocking Peptide

20-abx061096
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

GHITM Blocking Peptide

20-abx061097
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

SAR1B Blocking Peptide

20-abx061098
  • EUR 286.00
  • EUR 425.00
  • 1 mg
  • 5 mg

Cyclin A1 is therefore not only a large cellular cycle regulator with germinal-biased expression, essential for men and damaging for female meiosis, persistent expression during anaphase in oocytes shows fundamental differences between APC / C in early ecytes and embryos.

Karina

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