The FitAmp General Tissue Section DNA Isolation Kit is a comprehensive set of essential components that enables researchers to efficiently isolate DNA in any area of target microscopic tissue on a slide. It is suitable for isolating small amounts of DNA from microdissection samples, fresh tissue sections, formalin-fixed, and paraffin-embedded tissues, plasma, serum, body fluids, etc. Depending on the type of sample, the entire procedure can be completed in 2 hours.
Quick – The entire procedure can be completed in just 2 hours with consistent isolation conditions.
Efficient -Highly efficient isolation of DNA from tissue sections containing small amounts of DNA (as low as 1 ng).
Reliable – Our specially designed F-Spin columns allow DNA to be retrieved comfortably.
Safe: use of non-toxic reagents and without phenol-chloroform.
This kit can also be used as an initial step in the quantification of DNA methylation, followed by the use of the MethylFlash Global DNA Methylation 5-mC ELISA Easy Kit, or the MethylFlash Global DNA Methylation 5-hmC ELISA Easy Kit.
Fast and efficient DNA preparation is crucial for a successful DNA-based experiment. DNA samples can be prepared from cells, tissues, and body fluids, and are the most widely used starting material for most DNA methylation assays and applications. The FitAmp General Tissue Section DNA Isolation Kit ensures ready-to-use DNA no matter what the subsequent application.
Principle and procedure
This kit simply applies our proprietary DNA Isolation Buffer to a selected area of microscopic tissue. The area is removed and transferred to a tube. After treatment with DNA digestion buffer, DNA is easily recovered in 8-20 µl using our specially designed F-Spin column. DNA is ready for further application.
Materials to get started
The amount of input tissue can be as low as 1 ng with an optimal range between 10 ng and 1 µg. This kit can easily produce 1 ng to 4 µg of DNA in spinning column format, which can be used for any molecular biology procedure such as PCR, restriction digestion, cloning, and sequencing, etc.