Separase activity distribution can be a marker of major molecular response and proliferation of CD34+ cells in TKI-treated chronic myeloid leukemia patients.
Separase, a cysteine endopeptidase, are key players in the mitotic sister chromatid separation, the dynamics of the replication fork, and DNA repair. aberrant expression and / or modified separase proteolytic activity associated with aneuploidy, tumorigenesis, and progression of the disease. Due to genomic instability and clonal evolution of the hallmarks of the progress of chronic myeloid leukemia (CML), we have relatively examined separase proteolytic activity in TKI-treated chronic phase CML.
Separase proteolytic activity was analyzed at the single cell level in 88 clinical samples and in 14 healthy controls by flow cytometric assay. In parallel, the BCR-ABL1 gene expression and replication fork velocity was measured by qRT-PCR and DNA tests fiber, respectively. Separase Activity Distribution (SAD) value that indicates the occurrence separase MNC with increased proteolytic activity in the samples found to be positively correlated with BCR-ABL1 gene expression levels and the loss of MMR (relapses) throughout the routine monitoring of BCR-ABL1.
Analysis of CD34 + cells and MNC fractionated by flow cytometric cell sorting according to their level of activity separase (H- and L-fraction) revealed that the CD34 + cells with high activity levels separase (H-fraction) displayed increased proliferation / survival when compared with with normal cells (L-fraction) separase activity (average 3.3-fold, p = 0.0011). BCR-ABL1 positive effect gene expression in MNC H-fractions over L-fraction (42% vs. 8%, respectively).
In addition, expanded CD34 + cells from H-fraction showed a decrease in the speed of replication forks compared to cells L-fraction (p <0.0001). Our data show an association between high activity separase, the rest of the BCR-ABL1 gene expression, and increased capacity for proliferation in hematopoietic cells in leukemia niche TKI-treated chronic phase CML.
Separase activity distribution can be a marker of major molecular response and proliferation of CD34+ cells in TKI-treated chronic myeloid leukemia patients.
The data set on Separase-Sepin Inhibitor-1 toxicity in organ weights, hematology and clinical parameters in mice Sprague Dawley-.
Separase-Sepin Inhibitor-1 has shown great promise as a chemotherapeutic agent to treat Separase development-overexpressing tumors, however, very little is known about the toxicity profile. Here we present data set organ weights, hematology and clinical chemistry parameters in Sepin-1-treated rats Sprague Dawley-.
The data set is generated from two groups-Main Study Recovery Study and Study, life duration, in 29 and 57 days, respectively. Mice in both groups were covered with 0, 5, 10 and 20 mg / kg Sepin-1 once a day for 28 consecutive days. Blood samples from mice in the Main Study their organs were collected and weighed on day 29. The animals in the study were in a dose Recovery-off period of 28 days after being treated with Sepin-1 for 28 days, and samples of their blood and organ weight data were collected on day 57.
The mice’s body weights in both the Main and Recovery Study is collected twice a week. hematological parameters of samples of whole blood, such as hemoglobin concentration, platelet count and blood cells, etc., are determined. clinical chemistry parameters of serum, such as albumin concentration, glucose, cholesterol, triglycerides, alanine / aspartate aminotransferase, etc., are measured. Further analysis may yield useful information on the toxicity of Sepin-1 in Rat Sprague Dawley-. The data presented here relate to a research article entitled “Toxicity studies separase-Sepin inhibitor-1 in mice Sprague-Dowley”, available in Pathology – Research and Practice Journal
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801
Description: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids.
Description: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids.
Description: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ESPL1 / Separase (aa767-816). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801
Description: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates| sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1| or separase| which initiates the final separation of sister chromatids (Sun et al.| 2009
Description: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates| sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1| or separase| which initiates the final separation of sister chromatids (Sun et al.| 2009
Description: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates| sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1| or separase| which initiates the final separation of sister chromatids (Sun et al.| 2009
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Separase, a sister chromatid cohesion enzyme-finish, is an oncogene and is expressed in many human cancers. Sepin-1 (2,2-dimethyl-5-nitro-2H-benzimidazole-1,3-dioxide) is a potent inhibitor Separase which inhibit cancer cell growth, cell migration and wound healing, indicating that Sepin-1 has a great potential with a target separase-overexpressing tumors.
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