Increased separase activity and occurrence of centrosome aberrations concur with transformation of MDS.

Increased separase activity and occurrence of centrosome aberrations concur with transformation of MDS.

ESPL1 / separase, a cysteine ​​endopeptidase, is a key player in centrosome duplication and mitotic sister chromatid separation. aberrant expression and / or modified separase proteolytic activity associated with centrosome amplification, aneuploidy, tumorigenesis and progression of the disease. Since centrosome changes are common and early feature detected in patients with myelodysplastic syndrome (MDS) and the deviation cytogenetic play an important role in the risk stratification of disease, we tested the activity of separase at the level of single cells in the marrow samples 67 bone obtained from patients with MDS, secondary acute myeloid leukemia (SAML), de novo acute myeloid leukemia (AML) and healthy controls by flow cytometric separase activity test.

Separase Activity Distribution (SAD) value, calculated for the size of the cells with prominent separase activity in the samples analyzed, tested for correlation with the centrosome, karyotype and gene mutation status. We find the values ​​of SAD are higher in the patient’s bone marrow cells compared to the corresponding SAML MDS patients’ cells.

This concurs with the increased incidence of aberrant centrosome phenotype in MDS vs SAML samples. No correlation was found between the values ​​of SAD and mutation status karyotype / gen. During the follow-up of four MDS patients we observed an increase in the value of the SAD after transformation into SAML, two patients SAD value decreases during azacitidine therapy. cell culture experiments using cells of MDS-L as an in vitro model of MDS revealed that treatment with rigosertib, PLK1 inhibitors and therapeutic drugs known to induce G2 / M capture, results in a decrease in the value of SAD.

In conclusion, the appearance of cells with high levels of activity separase unusual, as indicated by the increased value of the SAD, agreed with the transformation of MDS to SAML and may reflect the potentially separase dysregulation contributes to clonal evolution during the progression of MDS. Separase measurement activities may therefore be useful as a new additional molecular markers for monitoring the disease.

Increased separase activity and occurrence of centrosome aberrations concur with transformation of MDS.
Increased separase activity and occurrence of centrosome aberrations concur with transformation of MDS.

Separase Detection Sensor Cleavage Live Event Using Mouse oocytes.

Separase proteolytic eliminate cohesin complex of sister chromatid arm in meiosis I, which are essential for chromosome segregation. Regulation separase activity is essential for proper cell cycle progression and proper chromosome segregation. Onset separase endogenous activity has not been observed in oocytes.

We live here describe a method for detecting separase activity in vivo mouse oocytes. This method utilizes previously described cleavage sensor consists of H2B-mCherry fused with Scc1 (aa 107-268) -YFP. Sensors division loaded on chromosome through the H2B-tag, and the signals from both mCherry and YFP visible. Upon activation separase Scc1 fragment cleaved and YFP dissociates from chromosomes. Changes in the ratio between mCherry and YFP fluorescence intensity is reading separase activity.


anaphase onset is irreversible cell cycle transitions triggered by protease activation Separase. Separase splitting Mcd1 (also known as Scc1) subunit of cohesin, a protein complex that physically connects the sister chromatids, triggering chromatid separation. Separase governed by degradation of anaphase inhibitor Securin freeing of inhibition of complex Separase Securin / Separase. In many organisms, Securin important not show that Separase governed by additional mechanisms.

Phospho-ESPL1 (S801) Antibody

MBS7115857-5x01mg 5x0.1mg
EUR 845

Phospho-MAP4K4 (S801) Antibody

E45M07149G-1 100 ul
EUR 395

Phospho-MAP4K4 (S801) Antibody

E45M07149G-4 50 ul
EUR 295

Phospho-MAP4K4 (S801) Antibody

MBS9201825-008mL 0.08mL
EUR 210

Phospho-MAP4K4 (S801) Antibody

MBS9201825-04mL 0.4mL
EUR 430

Phospho-MAP4K4 (S801) Antibody

MBS9201825-5x04mL 5x0.4mL
EUR 1910

Anti-Phospho-Separase (S801) antibody

STJ91032 200 µl
EUR 236.4
Description: Rabbit polyclonal to Phospho-Separase (S801).

Polyclonal Phospho-MAP4K4(S801) Antibody

AMM07149G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Phospho-MAP4K4(S801) . This antibody is tested and proven to work in the following applications:

Phospho-Separase (S801) Polyclonal Antibody

MBS2539730-002mL 0.02mL
EUR 140

Phospho-Separase (S801) Polyclonal Antibody

MBS2539730-006mL 0.06mL
EUR 180

Phospho-Separase (S801) Polyclonal Antibody

MBS2539730-012mL 0.12mL
EUR 260

Phospho-Separase (S801) Polyclonal Antibody

MBS2539730-02mL 0.2mL
EUR 405

Phospho-Separase (S801) Polyclonal Antibody

MBS2539730-5x02mL 5x0.2mL
EUR 1725

Anti-Phospho-Separase (S801) ESPL1 Antibody

A04364S801 100ul
EUR 380
Description: Boster Bio Anti-Phospho-Separase (S801) ESPL1 Antibody catalog # A04364S801. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Phospho-MAP4K4(S801) Antibody Blocking peptide

MBS9225138-INQUIRE INQUIRE Ask for price

S18-000003

555361 25.0mg
EUR 350

S18-000003

HY-119366 10 mg
EUR 1461.06
Description: S18-000003 is a potent, selective and orally active inhibitor of retinoic acid receptor-related orphan receptor-gamma-t (RORγt), with an IC50 of 10 μM). S18-000003 can be used for the research of psoriasis with low risk of thymic aberrations[1][2].

S18-000003

MBS3843562-10mg 10mg
EUR 810

S18-000003

MBS3843562-50mg 50mg
EUR 2370

S18-000003

MBS3843562-5mg 5mg
EUR 465

S18-000003

MBS3843562-5x50mg 5x50mg
EUR 10655

S18-000003

MBS5764698-10mg 10mg
EUR 405

S18-000003

MBS5764698-1mg 1mg
EUR 195

S18-000003

MBS5764698-25mg 25mg
EUR 730

S18-000003

MBS5764698-50mg 50mg
EUR 1125

S18-000003

MBS5764698-5mg 5mg
EUR 285

S18-000003

T16832-10mg 10mg Ask for price
Description: S18-000003

S18-000003

T16832-1g 1g Ask for price
Description: S18-000003

S18-000003

T16832-1mg 1mg Ask for price
Description: S18-000003

S18-000003

T16832-50mg 50mg Ask for price
Description: S18-000003

S18-000003

T16832-5mg 5mg Ask for price
Description: S18-000003

Gynkotek 320 340S 160 170S Lamp - EACH

LMP2016 EACH
EUR 591.3

S100 beta (S100 Calcium Binding Protein B, S100B, NEF, S100, S100beta) (AP)

MBS6436528-01mL 0.1mL
EUR 950

S100 beta (S100 Calcium Binding Protein B, S100B, NEF, S100, S100beta) (AP)

MBS6436528-5x01mL 5x0.1mL
EUR 4120

S100 beta (S100 Calcium Binding Protein B, S100B, NEF, S100, S100beta) (PE)

MBS6436538-01mL 0.1mL
EUR 950

S100 beta (S100 Calcium Binding Protein B, S100B, NEF, S100, S100beta) (PE)

MBS6436538-5x01mL 5x0.1mL
EUR 4120

S100 beta (S100 Calcium Binding Protein B, S100B, NEF, S100, S100beta) (APC)

MBS6436529-01mL 0.1mL
EUR 950

S100 beta (S100 Calcium Binding Protein B, S100B, NEF, S100, S100beta) (APC)

MBS6436529-5x01mL 5x0.1mL
EUR 4120

S100 beta (S100 Calcium Binding Protein B, S100B, NEF, S100, S100beta) (HRP)

MBS6436532-01mL 0.1mL
EUR 950

S100 beta (S100 Calcium Binding Protein B, S100B, NEF, S100, S100beta) (HRP)

MBS6436532-5x01mL 5x0.1mL
EUR 4120

S100 beta (S100 Calcium Binding Protein B, S100B, NEF, S100, S100beta) (FITC)

MBS6436531-01mL 0.1mL
EUR 950

S100 beta (S100 Calcium Binding Protein B, S100B, NEF, S100, S100beta) (FITC)

MBS6436531-5x01mL 5x0.1mL
EUR 4120

S07-2010

HY-150644 10 mg
EUR 573.6
Description: S07-2010 is a potent pan-AKR1C (aldo-keto reductase family 1 member C) inhibitor, with IC50 values of 0.19, 0.36, 0.47, and 0.73 μM for AKR1C3, AKR1C4, AKR1C1 and AKR1C2, respectively. S07-2010 induces apoptosis in A549/DDP cells. S07-2010 strengthens the cytotoxicity of chemotherapeutic agents in drug-resistant cells. S07-2010 significantly inhibits the proliferation of drug-resistant cells[1].

S07-2009

HY-150646 Get quote Ask for price
Description: S07-2009 is a potent and selective aldo-keto reductase 1C3 (AKR1C3) inhibitor with an IC50 value of 0.20 μM[1].

S07-2008

HY-150648 Get quote Ask for price
Description: S07-2008 is a selective aldo-keto reductase family 1 member C3 (AKR1C3) inhibitor with an IC50 of 0.16 μM. S07-2008 shows anticancer activities[1].

S07-2001

HY-150650 Get quote Ask for price
Description: S07-2001 is a potent and selective aldo-keto reductase 1C3 (AKR1C3) inhibitor with an IC50 value of 2.08 μM. S07-2001 enhances the activity of Doxorubicin against cancer cells. S07-2001 has potential as a chemotherapeutic potentiator for cancer agent resistance[1].

S19-1035

HY-152189 10 mg
EUR 367.97
Description: S19-1035 is a highly potent and specific aldo-keto reductase 1C3 (AKR1C3) inhibitor. S19-1035 inhibits AKR1C3 with an IC50 value of 3.04 nM. S19-1035 can be used for the research of tumor[1].

S07-1066

HY-155035 1 mg
EUR 914.52
Description: S07-1066 is an aldo-keto reductase 1C3 (AKR1C3) inhibitor, synergizing doxorubicin (DOX) cytotoxicity. S07-1066 selectively blocks AKR1C3-mediated reduction of DOX, and reverses the DOX resistance in overexpressing AKR1C3 cells[1].

S217879

HY-156065 Get quote Ask for price
Description: S217879 is a highly potent and selective NRF2 activator. S217879 disrupts the KEAP1-NRF2 interaction leading to robust NRF2 pathway activation. S217879 can be used for non-alcoholic steatohepatitis (NASH) research[1].

S116836

HY-123450 10 mg
EUR 3246.8
Description: S116836, a potent, orally active BCR-ABL tyrosine kinase inhibitor, blocks both wild-type as well as T315I Bcr-Abl. S116836 arrests the cells in the G0/G1 phase of cell cycle, induces apoptosis, increases ROS production, and decreases GSH production in BaF3/WT and BaF3/T315I cells. S116836 also inhibits SRC, LYN, HCK, LCK and BLK, and receptor tyrosine kinases such as FLT3, TIE2, KIT, PDGFR-β. Antitumor activies[1][2][3]. S116836 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

S-777469

HY-145153 10 mg
EUR 2110.42
Description: S-777469 is a selective and orally available cannabinoid type 2 receptor (CB2) agonist with a Ki of 36 nM. S-777469 significantly suppresses compound 48/80-induced scratching behavior in mice in a dose-dependent manner. S-777469 produces its antipruritic effects by inhibiting itch signal transmission through CB2 agonism[1][2].

S-23906-1

MBS5783980-5mg 5(mg
EUR 915

S-23906-1

MBS5783980-5x5mg 5x5(mg
EUR 3970

S116836

MBS5774496-10mg 10(mg
EUR 1020

S116836

MBS5774496-25mg 25mg
EUR 2075

S116836

MBS5774496-5x25mg 5x25mg
EUR 9185

S07-2009

T61003-10mg 10mg Ask for price
Description: S07-2009

S07-2009

T61003-1g 1g Ask for price
Description: S07-2009

S07-2009

T61003-1mg 1mg Ask for price
Description: S07-2009

S07-2009

T61003-50mg 50mg Ask for price
Description: S07-2009

S07-2009

T61003-5mg 5mg Ask for price
Description: S07-2009

S07-2001

T61033-10mg 10mg Ask for price
Description: S07-2001

In this work, we show that the yeast starter Separase activate Cdk1 (Esp1 in yeast) through phosphorylation to trigger the onset of anaphase. Esp1 activation by a protein phosphatase 2A regulatory subunit associated with Cdc55 (PP2ACdc55) and Slk19 spindle protein.

Karina

Related Posts

Structure and Function of the Separase-Securin Complex

Structure and Function of the Separase-Securin Complex

The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.

The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.

Stability and pharmacokinetics of separase inhibitor-sepin-1 in sprague-dawley rats.

Stability and pharmacokinetics of separase inhibitor-sepin-1 in sprague-dawley rats.

Identification of Bioactive Small Molecule Inhibitors of Separase.

Identification of Bioactive Small Molecule Inhibitors of Separase.

No Comment

Leave a Reply

Your email address will not be published.

February 2025
M T W T F S S
 12
3456789
10111213141516
17181920212223
2425262728  

Tags

Recent Posts

Categories