Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion

Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion

Tetraploidy, a common feature on cancer, results in the presence of extra centrosomes, which have been associated with chromosomal instability (CIN) and aneuploidy. Deregulation of the number of centrosomes trigger tumorigenesis. However, what supernumerary centrosomes evolved during the rise of tetraploid cells is still not explained.

Here, generate isogenic tetraploid clones in colorectal cancer and non-transformed cells, we showed that the tetraploid near-clones showed a significant increase in the number of centrosomes. In addition, we found that the centrosome area near-tetraploids is twice as large in the near-diploid. To evaluate whether centrosome clustering that happens, we analyzed the number of centrioles reveal amplification centrioles.

Although more than half of the near-tetraploids maintained in culture centrosome aberrations not present. To test whether the cells are increasingly lost centrioles after being near-tetraploid, we transiently transfected diploid cells with siRNA against ESPL1 / Separase, protease responsible for triggering anaphase, to generate new cells in near-tetraploid. Finally, using this model, we assess the number of centrioles at different time-points after tetraploidization found that near-tetraploids quickly centrosomes lose from time to time.

Taken together, these data indicate that although the majority of the cells reduces the supernumerary centrosomes after tetraploidization, a small percentage remained extra centrioles, potentially resulting in CIN.

Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion
Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion

Dun1, Chk2 kinase-related, is the central regulator of securin-separase dynamics during DNA damage signal.

DNA damage checkpoint stop cycle progression at G2 cells in response to genotoxic insults. Central to the implementation of cell cycle checkpoint arrest is induced stabilization securin-separase complex (yeast Pds1-Esp1). checkpoint kinase Chk1 and Chk2 (yeast Chk1 and Rad53) is expected to contribute to the stability of the critical-separase securin complex by phosphorylation securin, rendering it resistant to proteolytic breakdown by the anaphase promoting complex (APC).

Dun1, a Rad53 paralog associated with Chk2, are also important for the post-imposed arrest. Dun1 required for transcription-induced DNA damage DNA repair genes; However, its role in the implementation of cell cycle arrest is still unknown. Here, we show that Dun1 role in the arrest checkpoint independent of its involvement in the improvement of gene transcription.

Instead, Dun1 Pds1 necessary to prevent damage during DNA damage in cells Dun1-deficiency lowers Pds1, escape G2 arrest and undergoing mitotic checkpoint despite the presence of active Chk1 and Rad53. Interestingly, the proteolytic degradation of Pds1 the absence Dun1 mediated not by APC but by HECT domain containing E3 ligase Rsp5. Our results demonstrate regulatory scheme where Dun1 prevent chromosome segregation during DNA damage by inhibiting Rsp5-mediated proteolytic degradation of securin Pds1.

Separase Antibody

44415-50ul 50ul
EUR 224.4

SEPARASE antibody

70R-31054 100 ug
EUR 294
Description: Rabbit polyclonal SEPARASE antibody

Separase antibody

70R-51689 100 ul
EUR 286
Description: Purified Polyclonal Separase antibody

Separase Antibody

AF9195 200ul
EUR 420

Separase Antibody

AF9195-100ul 100ul
EUR 280

Separase Antibody

AF9195-200ul 200ul
EUR 350

Separase Antibody

20-abx215242
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  • 100 ug
  • 50 ug

Separase Antibody

20-abx121903
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  • 100 ul
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  • 30 ul

Separase Antibody

ABF9195 100 ug
EUR 525.6

SEPARASE antibody (Ser801)

70R-31053 100 ug
EUR 294
Description: Rabbit polyclonal SEPARASE antibody (Ser801)

Separase (pS801) Antibody

20-abx134111
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Rabbit Polyclonal Separase Antibody

TA309645 100 µl Ask for price

Separase Polyclonal Antibody

E20-74243 100ug
EUR 225
Description: Available in various conjugation types.

Separase Polyclonal Antibody

ABP52424-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ABP52424-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ABP52424-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Conjugated Antibody

C44415 100ul
EUR 476.4

Separase Polyclonal Antibody

E44H02168 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

SEPARASE (Ab-801) Antibody

E11-0086B 100μg
EUR 225
Description: Available in various conjugation types.

SEPARASE (Ab-801) Antibody

8B0086 50ug
EUR 368
Description: SEPARASE (Ab-801) Antibody

Separase (A795) polyclonal antibody

E43P0374 100ul
EUR 225
Description: Available in various conjugation types.

SEPARASE (Phospho-Ser801) Antibody

E11-0086A 100μg
EUR 225
Description: Available in various conjugation types.

SEPARASE (Phospho-Ser801) Antibody

8A0086 50ug
EUR 368
Description: SEPARASE (Phospho-Ser801) Antibody

Separase Rabbit Polyclonal Antibody

ES3423-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase Rabbit Polyclonal Antibody

ES3423-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Mouse Monoclonal Separase Antibody (XJ11-4D7)

TA309647 100 µl Ask for price

Polyclonal ESPL1 / Separase Antibody (aa767-816)

APR02989G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ESPL1 / Separase (aa767-816). This antibody is tested and proven to work in the following applications:

Mouse Monoclonal Separase Antibody (XJ11-1B12)

TA309646 100 µl Ask for price

Separase (phospho Ser801) Polyclonal Antibody

E20-60872 100ug
EUR 225
Description: Available in various conjugation types.

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

E44H00548 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Separase (Phospho-Ser801) Polyclonal Antibody

12353 100ul
EUR 319

Separase (Phospho-Ser801) Polyclonal Antibody

12353-100ul 100ul
EUR 302.4

Separase (Phospho-Ser801) Polyclonal Antibody

12353-50ul 50ul
EUR 224.4

Separase (Phospho-Ser801) Polyclonal Antibody

E012353 100μg/100μl
EUR 255
Description: Available in various conjugation types.

Separase (phospho Ser801) Rabbit Polyclonal Antibody

ES1518-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (phospho Ser801) Rabbit Polyclonal Antibody

ES1518-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (ESPL1) rabbit polyclonal antibody, Aff - Purified

AP32216PU-N 50 µl Ask for price

Separase (ESPL1) rabbit polyclonal antibody, Aff - Purified

AP20283PU-N 100 µg Ask for price

Separase Blocking Peptide

AF9195-BP 1mg
EUR 234

Separase Blocking Peptide

20-abx161616
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  • 1 mg
  • 5 mg

SEPARASE Cell ELISA Kit

abx595539-96tests 96 tests
EUR 764.4

SEPARASE Cell ELISA Kit

abx595539-100g 100 µg Ask for price

SEPARASE Cell ELISA Kit

abx595539-10g 10 µg
EUR 493.75

SEPARASE Cell ELISA Kit

abx595539-50g 50 µg Ask for price

Separase (pS801) Blocking Peptide

20-abx162232
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  • 5 mg

Separase (ESPL1) pSer1126 rabbit polyclonal antibody, Aff - Purified

AP12707PU-N 400 µl Ask for price

SEPARASE Cell-Based ELISA Kit

EKA50537-5x96T 5x96T
EUR 2122.78

SEPARASE Cell-Based ELISA Kit

EKA50537-96T 96T
EUR 446.9

Separase (phospho Ser801) Rabbit pAb

E2252078 100ul
EUR 225
Description: Available in various conjugation types.

Separase (ESPL1) (1866-1966) mouse monoclonal antibody, clone XJ11-1B12, Azide Free

AM26568AF-N 100 µg Ask for price

SEPARASE Colorimetric Cell-Based ELISA Kit

EKC1516 100ul
EUR 686.4

SEPARASE Colorimetric Cell-Based ELISA Kit (OKAG01028)

OKAG01028 96 Wells
EUR 715.2
Description: Description of target: ;Species reactivity: Human, Mouse;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:

SEPARASE (Phospho-Ser801) Colorimetric Cell-Based ELISA Kit

EKC2000 100ul
EUR 686.4

Anti-Separase Antibody

A04364 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Separase Antibody (ESPL1) detection. Tested with WB, IHC, IF in Human, Mouse.

Anti-Separase antibody

STJ95608 200 µl
EUR 236.4
Description: Rabbit polyclonal to Separase.

Addi platform separators (4 ea) Add 1.25 to platform separation - EACH

MIX7118 EACH
EUR 83.7

Additional platform separators (4 ea.). Adds 1.25" to platform separation

BR2000-SP 1 PC
EUR 54.38

Puck Separator

M-CBP-PST1 1 UNIT
EUR 340
Description: Puck Separator

Separator - EACH

INS6224 EACH
EUR 22.95

Separin (ESPL1) Antibody

20-abx326179
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  • 100 ug
  • 50 ug

Separin (ESPL1) Antibody

20-abx301075
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  • 100 ug
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  • 200 ug
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Separin (ESPL1) Antibody

20-abx211834
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  • 100 ul
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Separin (ESPL1) Antibody

20-abx212982
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  • 100 ul
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Separin (ESPL1) Antibody

abx301075-100g 100 µg
EUR 362.5

Separin (ESPL1) Antibody

abx301075-20g 20 µg
EUR 162.5

Separin (ESPL1) Antibody

abx301075-50g 50 µg
EUR 250

Separin (ESPL1) Antibody

abx212982-100l 100 µl
EUR 350

Separin (ESPL1) Antibody

abx212982-50l 50 µl
EUR 250

Separin (ESPL1) Antibody

abx211834-100l 100 µl
EUR 350

Separin (ESPL1) Antibody

abx211834-50l 50 µl
EUR 250

Separin (ESPL1) Antibody

abx326179-100g 100 µg
EUR 250

Separin (ESPL1) Antibody

abx326179-50g 50 µg
EUR 187.5

Rabbit anti-Separase Antibody

DL93405A-100ul 100 ul
EUR 299
Description: ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Rabbit anti-Separase Antibody

DL93405A-50ul 50 ul
EUR 209.3
Description: ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Rabbit anti-Separase Antibody

YLD7067-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to Separase

Rabbit anti-Separase Antibody

YLD7067-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to Separase

SEPARIN (pS1126) Antibody

abx031912-400ul 400 ul
EUR 627.6

SEPARIN (pS1126) Antibody

abx031912-80l 80 µl
EUR 343.2

SEPARIN (pS1126) Antibody

abx031912-400l 400 µl
EUR 525

Separator UB 200mm - EACH

SIE1104 EACH
EUR 142.47

Separin (ESPL1) Antibody (HRP)

20-abx316258
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Separin (ESPL1) Antibody (HRP)

abx316258-100g 100 µg
EUR 362.5

Separin (ESPL1) Antibody (HRP)

abx316258-20g 20 µg
EUR 162.5

Separin (ESPL1) Antibody (HRP)

abx316258-50g 50 µg
EUR 250

Phase Separator 25ml

CHR5384 PK100
EUR 152.76

Puck Separator Tools

M-CP-111-028 1 UNIT
EUR 305
Description: Puck Separator Tools

Puck Separator Tools

X-CC-116 1pc.
EUR 446.7

Mannose Separopore® 4B Cell Separation Kit

20840070-1 1 Kit
EUR 166.28

Separin (ESPL1) Antibody (FITC)

20-abx316259
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  • 100 ug
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Separin (ESPL1) Antibody (FITC)

abx316259-100g 100 µg
EUR 362.5

Separin (ESPL1) Antibody (FITC)

abx316259-20g 20 µg
EUR 162.5

Separin (ESPL1) Antibody (FITC)

abx316259-50g 50 µg
EUR 250

Separating Gel Buffer 4X

10530008-1 100 mL
EUR 38.93

Separating Gel Buffer 4X

10530008-2 250 mL
EUR 63.78

Separating Gel Buffer 4X

10530008-3 500 mL
EUR 86.02

Magnetic Separator

B23803 2 mL/200 uL/15 mL
EUR 291.6
Description: This Magnetic Separator is a proprietary machine, supportinga the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time.

Magnetic Separator

MagSep1-1 1/pk
EUR 132

Phase Separator 70ml - PK50

CHR5386 PK50
EUR 130.95

Phase Separator 150ml - PK25

CHR5388 PK25
EUR 163.35

BioMag® Flask Separator

84101S-1 1each
EUR 537

MiniTube Mag Separator

MTMS-16 mL
EUR 224
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Separin (ESPL1) Antibody (Biotin)

20-abx316260
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  • 100 ug
  • 1 mg
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Separin (ESPL1) Antibody (Biotin)

abx316260-100g 100 µg
EUR 362.5

Separin (ESPL1) Antibody (Biotin)

abx316260-20g 20 µg
EUR 162.5

Separin (ESPL1) Antibody (Biotin)

abx316260-50g 50 µg
EUR 250

Separating Funnel PP 500ml - PK2

FUN21060 PK2
EUR 105.3

Phase Separator 15ml - PK100

CHR5382 PK100
EUR 156.6

McDonald Separator - EACH

INS6226 EACH
EUR 18.9

Jenway Water Separator - EACH

FLA1034 EACH
EUR 207.9

50ml Separating Funnel - EACH

FUN4123 EACH
EUR 117.45

Magnetic Separation Rack

CM101 32 well (200 μl/well)
EUR 140

Magnetic Separation Rack

CM103 24 well (1.5 ml/well)
EUR 140

Separatory Funnel Pear 500ml - PK2

2394T/500 PK2
EUR 275.4

Separating Funnel Pear 1000ml - PK2

FUN20650 PK2
EUR 230.85

Pyrex 1L Separating Funnel - EACH

FUN4126 EACH
EUR 156.6

Pyrex 2L Separating Funnel - EACH

FUN4128 EACH
EUR 210.6

BioMag® 15ml/50ml Tube Separator

84102S-1 1each
EUR 1453

BioMag® 12 x 75mm Test Tube Separator

84104S-1 1each
EUR 1901

Separating Funnel Pear 50ml - EACH

FUN2081 EACH
EUR 83.7

Separating Funnel Pear 100ml - EACH

FUN2083 EACH
EUR 95.85

Separating Funnel Pear 250ml - EACH

FUN2085 EACH
EUR 101.25

2L P/Shaped Separating Funnel - EACH

FUN4310 EACH
EUR 233.55

BioMag® 96-Well Plate Separator

8MB4109S-1 1magnet
EUR 955

Separating Funnel Holder - EACH

STA1094 EACH
EUR 44.15

Magnetic Separator (Re-usable)

1999-1 each
EUR 319.2

Jenway Water Separator Large - EACH

FLA1036 EACH
EUR 252.45

FlexiMag Magnetic Separator, Jr.

FMJ-1000 mL
EUR 224
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

FlexiMag Magnetic Separator

FMS-1000 mL
EUR 311.5
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Pyrex 100ml Separating Funnel - EACH

FUN4120 EACH
EUR 117.45

Pyrex 250ml Separating Funnel - EACH

FUN4122 EACH
EUR 128.25

Pyrex 500ml Separating Funnel - EACH

FUN4124 EACH
EUR 135

Funnel Pyrex Separating Pear 5L - EACH

FUN4312 EACH
EUR 348.3

Cardboard Separator kit for xxxx - PK6

CRY2076 PK6
EUR 29.7

Separating funnel 100ML PTFE Key - EACH

8S149/100 EACH
EUR 51.3

Separating Funnel 500ml PTFE Key - EACH

8S149500 EACH
EUR 63.45

MicroMag Magnetic Separator

MMS-2100 mL
EUR 262.5
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

HandiMag Magnetic Separator

HMS-1000 mL
EUR 101.5
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

UltraMag DW Magnetic Separator

UMDS-1000 mL
EUR 402.5
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

UltraMag Magnetic Separator

UMS-3000 mL
EUR 399
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

One.Step RT-PCR Kit (5X) with separate ROX / 20 µl tests

106-100 200 rcs
EUR 249

One.Step RT-PCR Kit (5X) with separate ROX / 20 µl tests

106-100L 2000rcs
EUR 1599

One.Step RT-PCR Kit (5X) with separate ROX / 20 µl tests

106-100XL 10000rcs Ask for price

50ml P/Shaped Separating Funnel - EACH

FUN4300 EACH
EUR 124.2

Long Form Separating Funnel 50ml - EACH

8S14950 EACH
EUR 49.95

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200125 PK100
EUR 64.41

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200150 PK100
EUR 90.87

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200185 EACH
EUR 91.08

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200240 EACH
EUR 127.71

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200270 EACH
EUR 197.01

Nalgene 125ml Separating Funnel PP - EACH

FUN4180 EACH
EUR 109.35

T-Pro Separating or Resolving Buffer

JB03-C001 500ml/BT
EUR 182.4

EpiMag HT (96-Well) Magnetic Separator

Q10002
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  • 1/Pack
  • 1/Pack

Bromoform for Mineral Separation

GRM10947-250ML 1 unit
EUR 66.62
Description: Bromoform for Mineral Separation

genesig magnetic separation rack

Z-genesigEASY-MR n/a
EUR 539

Rabbit anti-Separase (pS801) Antibody

DL92370A-100ul 100 ul
EUR 299
Description: ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Rabbit anti-Separase (pS801) Antibody

DL92370A-50ul 50 ul
EUR 209.3
Description: ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Rabbit anti-Separase (pS801) Antibody

YLD7066-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to Separase (pS801)

Rabbit anti-Separase (pS801) Antibody

YLD7066-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to Separase (pS801)

Anti-Phospho-Separase (S801) antibody

STJ91032 200 µl
EUR 236.4
Description: Rabbit polyclonal to Phospho-Separase (S801).

Culture Tube, 14mL, 17 x 100mm, PP, w/ separate 2-position screw-cap, non-sterile, non-graduated, 1000 tubes and capped bulk packed separately

T8850 1000/pack
EUR 223.63
Description: Culture Tube 14mL

Culture Tube, 5mL, 12 x 75mm, PP, w/ separate 2-position screw-cap, non-sterile, non-graduated, 1000 tubes and capped bulk packed separately

T8750 1000/pack
EUR 130.8
Description: Culture Tube 5mL

Lymphocyte Separation Solution

20828-15 500ML
EUR 207.9

Lymphocyte Separation Solution

20828-44 100ML
EUR 46.2

Lymphocyte Separation Solution

20839-04 100ML
EUR 57.4

100 ML LSM (LYMPHOCYTE SEPARATION MEDIUM)

25-072-CI 100 mL/pk
EUR 63.6
Description: Media Catalog; Cell Culture Reagents

Statebourne 1 2 BSP Phase Separator - EACH

CRY2104 EACH
EUR 165.97

PCR 8-Strips, 0.2ml with Separate Flat Cap Strips, 120/pk

P3010-S 120/pack
EUR 82.68
Description: PCR 8-Strips 0.2ml with Separate Flat Cap Strips

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA - EACH

WHA2200070 EACH
EUR 51.3

Cytosol/Particulate Separation Kit

55R-1356 50 assays
EUR 433
Description: Cytosol/Particulate Separation Kit for use in the research laboratory


Often resulting in prolonged mitotic apoptosis1. Abbreviated tumorigenic mitosis causing aneuploidy, but it is not clear whether it also activates apoptosis machinery2. Separase, cysteine ​​proteases and triggers of all anaphases eukaryotic, have caspase-like catalytic domains but have never been associated with cell death3,4. Here we show that human cells entering mitosis with separase already active to quickly undergo mitosis death because cleavage directly MCL1 and anti-apoptotic BCL-XL by separase. Cleavage is not only prevent MCL1 and BCL-XL from the execution of pro-apoptotic BAK, but also convert them into active promoters of death in mitosis.

Karina

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