
Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion
Tetraploidy, a common feature on cancer, results in the presence of extra centrosomes, which have been associated with chromosomal instability (CIN) and aneuploidy. Deregulation of the number of centrosomes trigger tumorigenesis. However, what supernumerary centrosomes evolved during the rise of tetraploid cells is still not explained.
Here, generate isogenic tetraploid clones in colorectal cancer and non-transformed cells, we showed that the tetraploid near-clones showed a significant increase in the number of centrosomes. In addition, we found that the centrosome area near-tetraploids is twice as large in the near-diploid. To evaluate whether centrosome clustering that happens, we analyzed the number of centrioles reveal amplification centrioles.
Although more than half of the near-tetraploids maintained in culture centrosome aberrations not present. To test whether the cells are increasingly lost centrioles after being near-tetraploid, we transiently transfected diploid cells with siRNA against ESPL1 / Separase, protease responsible for triggering anaphase, to generate new cells in near-tetraploid. Finally, using this model, we assess the number of centrioles at different time-points after tetraploidization found that near-tetraploids quickly centrosomes lose from time to time.
Taken together, these data indicate that although the majority of the cells reduces the supernumerary centrosomes after tetraploidization, a small percentage remained extra centrioles, potentially resulting in CIN.

Dun1, Chk2 kinase-related, is the central regulator of securin-separase dynamics during DNA damage signal.
DNA damage checkpoint stop cycle progression at G2 cells in response to genotoxic insults. Central to the implementation of cell cycle checkpoint arrest is induced stabilization securin-separase complex (yeast Pds1-Esp1). checkpoint kinase Chk1 and Chk2 (yeast Chk1 and Rad53) is expected to contribute to the stability of the critical-separase securin complex by phosphorylation securin, rendering it resistant to proteolytic breakdown by the anaphase promoting complex (APC).
Dun1, a Rad53 paralog associated with Chk2, are also important for the post-imposed arrest. Dun1 required for transcription-induced DNA damage DNA repair genes; However, its role in the implementation of cell cycle arrest is still unknown. Here, we show that Dun1 role in the arrest checkpoint independent of its involvement in the improvement of gene transcription.
Instead, Dun1 Pds1 necessary to prevent damage during DNA damage in cells Dun1-deficiency lowers Pds1, escape G2 arrest and undergoing mitotic checkpoint despite the presence of active Chk1 and Rad53. Interestingly, the proteolytic degradation of Pds1 the absence Dun1 mediated not by APC but by HECT domain containing E3 ligase Rsp5. Our results demonstrate regulatory scheme where Dun1 prevent chromosome segregation during DNA damage by inhibiting Rsp5-mediated proteolytic degradation of securin Pds1.
SEPARASE antibody |
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70R-31054 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal SEPARASE antibody |
Separase Antibody |
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AF9195 | Affbiotech | 200ul | EUR 420 |
Separase antibody |
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70R-51689 | Fitzgerald | 100 ul | EUR 344.4 |
Description: Purified Polyclonal Separase antibody |
Separase Antibody |
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ABF9195 | Lifescience Market | 100 ug | EUR 525.6 |
SEPARASE antibody (Ser801) |
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70R-31053 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal SEPARASE antibody (Ser801) |
Separase (pS801) Antibody |
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20-abx134111 | Abbexa |
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Separase Conjugated Antibody |
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C44415 | SAB | 100ul | EUR 476.4 |
Separase Polyclonal Antibody |
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ABP52424-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801 |
Separase Polyclonal Antibody |
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ABP52424-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801 |
Separase Polyclonal Antibody |
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ABP52424-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801 |
Separase Polyclonal Antibody |
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ES3423-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
Separase Polyclonal Antibody |
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ES3423-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
Polyclonal ESPL1 / Separase Antibody (aa767-816) |
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APR02989G | Leading Biology | 0.05ml | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ESPL1 / Separase (aa767-816). This antibody is tested and proven to work in the following applications: |
Separase (phospho Ser801) Polyclonal Antibody |
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ABP50519-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801 |
Separase (phospho Ser801) Polyclonal Antibody |
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ABP50519-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801 |
Separase (phospho Ser801) Polyclonal Antibody |
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ABP50519-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801 |
Separase (phospho Ser801) Polyclonal Antibody |
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ES1518-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
Separase (phospho Ser801) Polyclonal Antibody |
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ES1518-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
Separase (Phospho-Ser801) Polyclonal Antibody |
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12353-100ul | SAB | 100ul | EUR 302.4 |
Separase (Phospho-Ser801) Polyclonal Antibody |
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12353-50ul | SAB | 50ul | EUR 224.4 |
Separase Blocking Peptide |
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AF9195-BP | Affbiotech | 1mg | EUR 234 |
Separase Blocking Peptide |
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20-abx161616 | Abbexa |
|
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SEPARASE Cell ELISA Kit |
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abx595539-96tests | Abbexa | 96 tests | EUR 764.4 |
Separase (pS801) Blocking Peptide |
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20-abx162232 | Abbexa |
|
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SEPARASE Colorimetric Cell-Based ELISA Kit |
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EKC1516 | BosterBio | 100ul | EUR 686.4 |
SEPARASE Colorimetric Cell-Based ELISA Kit (OKAG01028) |
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OKAG01028 | Aviva Systems Biology | 96 Wells | EUR 715.2 |
Description: Description of target: ;Species reactivity: Human, Mouse;Application: ELISA;Assay info: Assay Type: Cell-Based Subtype: None Detection Method: Colorimetric 450 nm;Sensitivity: |
SEPARASE (Phospho-Ser801) Colorimetric Cell-Based ELISA Kit |
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EKC2000 | BosterBio | 100ul | EUR 686.4 |
Addi platform separators (4 ea) Add 1.25 to platform separation |
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MIX7118 | Scientific Laboratory Supplies | EACH | EUR 74.4 |
Separator |
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INS6224 | Scientific Laboratory Supplies | EACH | EUR 20.4 |
Additional platform separators (4 ea.). Adds 1.25" to platform separation |
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BR2000-SP | Benchmark Scientific | 1 PC | EUR 52.8 |
Separin (ESPL1) Antibody |
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20-abx212982 | Abbexa |
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Separin (ESPL1) Antibody |
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20-abx211834 | Abbexa |
|
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Separin (ESPL1) Antibody |
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20-abx326179 | Abbexa |
|
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Separin (ESPL1) Antibody |
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20-abx301075 | Abbexa |
|
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Anti-Separase Antibody |
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A04364 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for Separase Antibody (ESPL1) detection. Tested with WB, IHC, IF in Human, Mouse. |
Anti-Separase antibody |
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STJ95608 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Separase. |
Puck Separator |
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M-CBP-PST1 | MiTeGen | 1 UNIT | EUR 340 |
Description: Puck Separator |
Separator UB 200mm |
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SIE1104 | Scientific Laboratory Supplies | EACH | EUR 126.64 |
SEPARIN (pS1126) Antibody |
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abx031912-400ul | Abbexa | 400 ul | EUR 627.6 |
SEPARIN (pS1126) Antibody |
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abx031912-80l | Abbexa | 80 µl | EUR 343.2 |
Separin (ESPL1) Antibody (HRP) |
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20-abx316258 | Abbexa |
|
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Separin (ESPL1) Antibody (FITC) |
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20-abx316259 | Abbexa |
|
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Phase Separator 15ml |
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CHR5382 | Scientific Laboratory Supplies | PK100 | EUR 139.2 |
Phase Separator 25ml |
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CHR5384 | Scientific Laboratory Supplies | PK100 | EUR 152.76 |
Phase Separator 70ml |
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CHR5386 | Scientific Laboratory Supplies | PK50 | EUR 116.4 |
Phase Separator 150ml |
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CHR5388 | Scientific Laboratory Supplies | PK25 | EUR 145.2 |
Puck Separator Tools |
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M-CP-111-028 | MiTeGen | 1 UNIT | EUR 305 |
Description: Puck Separator Tools |
Magnetic Separator |
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B23803 | Bimake | 2 mL/200 uL/15 mL | EUR 291.6 |
Description: This Magnetic Separator is a proprietary machine, supportinga the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time. |
Magnetic Separator |
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MagSep1-1 | ABTBeads | 1/pk | EUR 132 |
McDonald Separator |
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INS6226 | Scientific Laboratory Supplies | EACH | EUR 16.8 |
Separin (ESPL1) Antibody (Biotin) |
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20-abx316260 | Abbexa |
|
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MiniTube Mag Separator |
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MTMS-16 | Spherotech | mL | EUR 432 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
Jenway Water Separator |
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FLA1034 | Scientific Laboratory Supplies | EACH | EUR 184.8 |
Separating Funnel PP 500ml |
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FUN21060 | Scientific Laboratory Supplies | PK2 | EUR 93.6 |
50ml Separating Funnel |
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FUN4123 | Scientific Laboratory Supplies | EACH | EUR 104.4 |
Pyrex 1L Separating Funnel |
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FUN4126 | Scientific Laboratory Supplies | EACH | EUR 139.2 |
Pyrex 2L Separating Funnel |
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FUN4128 | Scientific Laboratory Supplies | EACH | EUR 187.2 |
Separatory Funnel Pear 500ml |
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2394T/500 | Scientific Laboratory Supplies | PK2 | EUR 244.8 |
Magnetic Separation Rack |
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M1501-1 | Biovision | 1 Rack | EUR 454.8 |
Separating Funnel Pear 1000ml |
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FUN20650 | Scientific Laboratory Supplies | PK2 | EUR 205.2 |
Separating Funnel Pear 50ml |
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FUN2081 | Scientific Laboratory Supplies | EACH | EUR 74.4 |
Separating Funnel Pear 100ml |
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FUN2083 | Scientific Laboratory Supplies | EACH | EUR 85.2 |
Separating Funnel Pear 250ml |
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FUN2085 | Scientific Laboratory Supplies | EACH | EUR 90 |
2L P/Shaped Separating Funnel |
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FUN4310 | Scientific Laboratory Supplies | EACH | EUR 207.6 |
Separating Funnel Holder |
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STA1094 | Scientific Laboratory Supplies | EACH | EUR 39.24 |
Magnetic Separation Rack |
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CM101 | Vazyme | 32 well (200 μl/well) | EUR 112 |
Magnetic Separation Rack |
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CM103 | Vazyme | 24 well (1.5 ml/well) | EUR 112 |
Separating funnel 100ML PTFE Key |
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8S149/100 | Scientific Laboratory Supplies | EACH | EUR 45.6 |
Separating Funnel 500ml PTFE Key |
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8S149500 | Scientific Laboratory Supplies | EACH | EUR 56.4 |
Jenway Water Separator Large |
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FLA1036 | Scientific Laboratory Supplies | EACH | EUR 224.4 |
Pyrex 100ml Separating Funnel |
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FUN4120 | Scientific Laboratory Supplies | EACH | EUR 104.4 |
Pyrex 250ml Separating Funnel |
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FUN4122 | Scientific Laboratory Supplies | EACH | EUR 114 |
Pyrex 500ml Separating Funnel |
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FUN4124 | Scientific Laboratory Supplies | EACH | EUR 120 |
Funnel Pyrex Separating Pear 5L |
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FUN4312 | Scientific Laboratory Supplies | EACH | EUR 309.6 |
Cardboard Separator kit for xxxx |
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CRY2076 | Scientific Laboratory Supplies | PK6 | EUR 26.4 |
Long Form Separating Funnel 50ml |
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8S14950 | Scientific Laboratory Supplies | EACH | EUR 44.4 |
50ml P/Shaped Separating Funnel |
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FUN4300 | Scientific Laboratory Supplies | EACH | EUR 110.4 |
Magnetic Separator (Re-usable) |
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1999-1 | Biovision | each | EUR 319.2 |
MicroMag Magnetic Separator |
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MMS-2100 | Spherotech | mL | EUR 498 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
FlexiMag Magnetic Separator |
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FMS-1000 | Spherotech | mL | EUR 583.2 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
FlexiMag Magnetic Separator |
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FMJ-1000 | Spherotech | mL | EUR 432 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
Nalgene 125ml Separating Funnel PP |
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FUN4180 | Scientific Laboratory Supplies | EACH | EUR 97.2 |
HandiMag Magnetic Separator |
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HMS-1000 | Spherotech | mL | EUR 218.4 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
UltraMag DW Magnetic Separator |
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UMDS-1000 | Spherotech | mL | EUR 741.6 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
UltraMag Magnetic Separator |
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UMS-3000 | Spherotech | mL | EUR 736.8 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
Statebourne 1 2 BSP Phase Separator |
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CRY2104 | Scientific Laboratory Supplies | EACH | EUR 147.53 |
WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA |
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WHA2200070 | Scientific Laboratory Supplies | EACH | EUR 45.6 |
WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA |
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WHA2200110 | Scientific Laboratory Supplies | PK100 | EUR 60 |
WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA |
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WHA2200125 | Scientific Laboratory Supplies | PK100 | EUR 68.4 |
WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA |
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WHA2200150 | Scientific Laboratory Supplies | PK100 | EUR 96 |
WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA |
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WHA2200185 | Scientific Laboratory Supplies | EACH | EUR 110.4 |
WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA |
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WHA2200240 | Scientific Laboratory Supplies | EACH | EUR 154.8 |
WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA |
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WHA2200270 | Scientific Laboratory Supplies | EACH | EUR 238.8 |
T-Pro Separating or Resolving Buffer |
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JB03-C001 | T-Pro Biotechnology | 500ml/BT | EUR 182.4 |
EpiMag HT (96-Well) Magnetic Separator |
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Q10002 | EpiGentek |
|
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WHATMAN(R) TG100 SEPARATION GAUZE DIAM |
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WHA10423029 | Scientific Laboratory Supplies | EACH | EUR 174 |
100 ML LSM (LYMPHOCYTE SEPARATION MEDIUM) |
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25-072-CI | CORNING | 100 mL/pk | EUR 63.6 |
Description: Media Catalog; Cell Culture Reagents |
Separator type 10001 for drawers capacity 10000 cm3 |
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DD039473 | Scientific Laboratory Supplies | EACH | EUR 7.2 |
PCR 8-Strips, 0.2ml with Separate Flat Cap Strips, 120/pk |
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P3010-S | MTC Bio | 120/pack | EUR 116.7 |
Description: PCR 8-Strips 0.2ml with Separate Flat Cap Strips |
Culture Tube, 5mL, 12 x 75mm, PP, w/ separate 2-position screw-cap, non-sterile, non-graduated, 1000 tubes and capped bulk packed separately |
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T8750 | MTC Bio | 1000/pack | EUR 147.48 |
Description: Culture Tube 5mL |
Culture Tube, 14mL, 17 x 100mm, PP, w/ separate 2-position screw-cap, non-sterile, non-graduated, 1000 tubes and capped bulk packed separately |
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T8850 | MTC Bio | 1000/pack | EUR 228.48 |
Description: Culture Tube 14mL |
Lymphocyte separation medium (human) |
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abx082102-200ml | Abbexa | 200 ml | EUR 243.6 |
Cytosol/Particulate Separation Kit |
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55R-1356 | Fitzgerald | 50 assays | EUR 661.2 |
Description: Cytosol/Particulate Separation Kit for use in the research laboratory |
Cytosol/Particulate Separation Kit |
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K267-50 | Biovision | each | EUR 483.6 |
Cytosol/Particulate Separation Kit |
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K2112-50 | ApexBio | 50 assays | EUR 501.6 |
WHATMAN 1PS PHASE SEPARATOR FILTER PAPER |
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WHA2200090 | Scientific Laboratory Supplies | EACH | EUR 49.2 |
1-Step Polymorphs, Human Cell Separation |
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71-AN221725 | Gentaur Genprice | 1 | EUR 238.8 |
Taq DNA Polymerase - 5,000 units with separate dNTP Mix |
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3245d | Intact Genomics | 1/EA | EUR 802.8 |
Separin Phospho-Ser801 (ESPL1 pS801) Antibody |
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20-abx326180 | Abbexa |
|
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Techne Separation Membranes 200x200mm |
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OVE3038 | Scientific Laboratory Supplies | PK5 | EUR 181.2 |
Custom drilling for BSW01 (sold separately) up to 36 holes |
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BLO1274 | Scientific Laboratory Supplies | EACH | EUR 260.4 |
Custom drilling for BSW01 (sold separately), up to 36 holes |
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BSW01-DR | Benchmark Scientific | 1 PC | EUR 195.3 |
Funnel Separating Conical Ptfe Stopcock 100ml |
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E4606 | Scientific Laboratory Supplies | EACH | EUR 33.6 |
Funnel Separating Conical Ptfe Stopcock 250ml |
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E4607 | Scientific Laboratory Supplies | EACH | EUR 43.2 |
Orion Star A211 pH Benchtop Meter Separate Sensor Kit |
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STARA2118 | Scientific Laboratory Supplies | EACH | EUR 1358.4 |
Mercury is supplied as a separate solution (PLHG-1Y). |
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TCLP-100 | Scientific Laboratory Supplies | 125ML | EUR 253.2 |
Mercury is supplied as a separate solution (PLHG-1X). |
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TCLP-500 | Scientific Laboratory Supplies | 500ML | EUR 429.6 |
Stacking platform, small 10.5"x7.5" with flat mat (3.0"separation) |
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B3D-STACK | Benchmark Scientific | 1 PC | EUR 135.8 |
Corning 500mL LSM (Lymphocyte Separation Medium) |
|||
25-072-CV | Scientific Laboratory Supplies | EACH | EUR 156 |
Clear Glass Powder Bottle with Separate Black Cap - 15mL |
|||
BOT1200 | Scientific Laboratory Supplies | PK140 | EUR 87.6 |
Clear Glass Powder Bottle with Separate Black Cap - 30mL |
|||
BOT1201 | Scientific Laboratory Supplies | PK80 | EUR 49.2 |
Clear Glass Powder Bottle with Separate Black Cap - 60mL |
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BOT1204 | Scientific Laboratory Supplies | PK72 | EUR 50.4 |
Clear Glass Powder Bottle with Separate Black Cap - 125mL |
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BOT1206 | Scientific Laboratory Supplies | PK80 | EUR 60 |
Clear Glass Powder Bottle with Separate Black Cap - 250mL |
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BOT1211 | Scientific Laboratory Supplies | PK42 | EUR 37.2 |
Clear Glass Powder Bottle with Separate Black Cap - 500mL |
|||
BOT1212 | Scientific Laboratory Supplies | PK33 | EUR 50.4 |
Amber Glass Powder Bottle with Separate Black Cap - 30mL |
|||
BOT1220 | Scientific Laboratory Supplies | PK127 | EUR 81.6 |
Amber Glass Powder Bottle with Separate Black Cap - 60mL |
|||
BOT1222 | Scientific Laboratory Supplies | PK72 | EUR 40.8 |
Amber Glass Powder Bottle with Separate Black Cap - 250mL |
|||
BOT1226 | Scientific Laboratory Supplies | PK42 | EUR 74.4 |
Amber Glass Powder Bottle with Separate Black Cap - 500mL |
|||
BOT1228 | Scientific Laboratory Supplies | PK28 | EUR 39.6 |
Mercury is supplied as a separate solution (PLHG-1AX). |
|||
EP-8-500 | Scientific Laboratory Supplies | 500ML | EUR 366 |
Mercury is supplied as a separate solution (PLHG2-1Y). |
|||
LPC-1-100 | Scientific Laboratory Supplies | 125ML | EUR 528 |
Mercury is supplied as a separate solution (PLHG2-1X). |
|||
LPC-1-500 | Scientific Laboratory Supplies | 500ML | EUR 912 |
0.1ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), WHITE 120/pk |
|||
P3801-QW | MTC Bio | 120/pack | EUR 162.06 |
Description: 0.1ml qPCR 8-Strip ,white 120 |
0.2ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), WHITE, 120/pk |
|||
P3802-QW | MTC Bio | 120/pack | EUR 162.06 |
Description: 0.2ml qPCR 8-Strip , white |
Stacking platform small 10.5x7.5 w flat mat (3.0separation) |
|||
MIX7112 | Scientific Laboratory Supplies | EACH | EUR 182.4 |
Simax Separating Funnel Cylindrical Open 50ml |
|||
FUN2060 | Scientific Laboratory Supplies | EACH | EUR 46.8 |
Simax Separating Funnel Cylindrical Open 100ml |
|||
FUN20660 | Scientific Laboratory Supplies | PK4 | EUR 180 |
Pyrex Separating Funnel 100ml Glass Stopcock Pear |
|||
FUN4322 | Scientific Laboratory Supplies | EACH | EUR 117.6 |
Mercury is supplied as a separate solution (PLHG2-1Y). |
|||
INTER18-100 | Scientific Laboratory Supplies | 125ML | EUR 501.6 |
Mercury is supplied as a separate solution (PLHG2-1X). |
|||
INTER18-500 | Scientific Laboratory Supplies | 500ML | EUR 874.8 |
Mercury is supplied as a separate solution (PLHG2-AY). |
|||
WP-15 | Scientific Laboratory Supplies | 125ML | EUR 328.8 |
Stacking platform, large 12"x12" with flat mat (3.0" separation) |
|||
BR1000-STACK | Benchmark Scientific | 1 PC | EUR 135.8 |
Mercury is supplied as a separate solution (PLHG2-1AY). |
|||
EP-8 | Scientific Laboratory Supplies | 125ML | EUR 220.8 |
PCR Tubes Strips of 8 tubes w/ dome caps packaged separately, 120/pk |
|||
P3020 | MTC Bio | 120/pack | EUR 118.32 |
Description: PCR Tubes Strips of 8 tubes w/ dome caps packaged separately120 |
Stacking platform large 12x12 w flat mat (3.0 separation) |
|||
MIX7114 | Scientific Laboratory Supplies | EACH | EUR 182.4 |
Mercury is supplied as a separate solution (PLHG2-1AX). |
|||
WP-15-500 | Scientific Laboratory Supplies | 500ML | EUR 550.8 |
0.1ml qPCR 8-Strip (With SEPARATE Optical Strip Caps) FROSTED, 120/pk |
|||
P3801-QF | MTC Bio | 120/pack | EUR 157.2 |
Description: 0.1ml qPCR 8-Strip , frosted, 120 |
Polar/Neutral Lipid Separation Kit (Chloroform Free) |
|||
MET-5009 | Cell Biolabs | 50 preps | EUR 428.4 |
Description: Our Polar/Neutral Lipid Separation Kit separates polar from neutral lipids starting from a total organic lipid pool obtained through extraction by the Folch method or our Lipid Extraction Kit. Polar and neutral lipid separation occurs through the addition of a proprietary secondary alcohol and a proprietary secondary organic compound. After phase separation, the top layer containing the neutral lipids is separated from polar lipids in the bottom layer. The layers are dried and the lipidsresuspended for downstream analysis. |
Stacking platform, small 10.5"x7.5" with dimpled mat (3.0" separation) |
|||
B3D-STACK-D | Benchmark Scientific | 1 PC | EUR 141 |
5mL Tubes, non-sterile, w/ screw caps packed separately, pk/500 ( 5 bags of 100) |
|||
C1005-SC5 | Benchmark Scientific | 1 each | EUR 99 |
Pyrex Morbank Separating Funnel 1L Glass Stopcock Pear |
|||
FUN4328 | Scientific Laboratory Supplies | EACH | EUR 148.8 |
Anti-Phospho-Separase (S801) antibody |
|||
STJ91032 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Phospho-Separase (S801). |
Stacking platform, large 12"x12" with dimpled mat (3.0" separation) |
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BR1000-STACK-D | Benchmark Scientific | 1 PC | EUR 141 |
Stacking Platform, extra large 14"x12" with flat mat (3.0" separation) |
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BR2000-STACK | Benchmark Scientific | 1 PC | EUR 147.7 |
OrgFrontier? Viable/Non-Viable Cells Separation Kit |
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K850-10 | Biovision | each | EUR 652.8 |
Stacking platform large 12x12 w dimpled mat (3.0 separation) |
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MIX7116 | Scientific Laboratory Supplies | EACH | EUR 188.4 |
0.1ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), Natural/Clear, 120/pk |
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P3801-Q | MTC Bio | 120/pack | EUR 157.2 |
Description: 0.1ml qPCR 8-Strip , Natural/Clear |
0.2ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), Natural/Clear, 120/pk |
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P3802-Q | MTC Bio | 120/pack | EUR 157.2 |
Description: 0.2ml qPCR 8-Strip , Natural/Clear |
Pyrex Pear Shaped Separating Funnel with PTFE Stopcock 250ml |
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FUN4324 | Scientific Laboratory Supplies | EACH | EUR 122.4 |
Custom drilling for BCM1412-BSW01 (sold separately), up to 36 holes |
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BCM1412-BSW01-DR | Bio Basic | 1 pcs, 1 UNIT | EUR 289.42 |
Brand squibb separating funnel, pp stopper, ungraduated, ptfe key, 250 ml |
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Z330930-2EA | Scientific Laboratory Supplies | PK2 | EUR 165.81 |
5mL Screw-Cap MacroTube, non-sterile, w/ screw caps packed separately |
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C2530 | MTC Bio | 500/pack | EUR 139.38 |
Description: 5mL MacroTubes |
Shaking Platform Pre-drilled For Flask Clamps (clamps Sold Separately) |
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480159 | Scientific Laboratory Supplies | EACH | EUR 430.8 |
Grant-Bio Orbital Shaker for Use in CO2 Incubators - Platforms Sold Separately |
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SHA7830 | Scientific Laboratory Supplies | EACH | EUR 1984.8 |
Grant-Bio Overhead Stirrer Multi Mixer - Attachments Sold Separately |
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STI1270 | Scientific Laboratory Supplies | EACH | EUR 732 |
Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free) |
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MET-5009-C | Cell Biolabs | 50 preps | EUR 790.8 |
Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis. |
LEXSY Cultivation Kit P10 ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight |
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M-LT-102 | MiTeGen | 1 Kit | EUR 1279 |
Description: LEXSY Cultivation Kit P10 ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight |
LEXSY Cultivation Kit T7-TR ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight |
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M-LT-111 | MiTeGen | 1 Kit | EUR 2841 |
Description: LEXSY Cultivation Kit T7-TR ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight |
TARGATT ? Transgenic Kit (5 microinjections) Academic Price *Plasmid sold separately |
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AST-1003 | Applied StemCell | 1 Kit (5 microinjections) | EUR 691.8 |
Description: 6 month |
TARGATT ? Transgenic Kit (2 microinjections) Academic Price *Plasmid sold separately |
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AST-1004 | Applied StemCell | 1 Kit (2 microinjections) | EUR 388.06 |
Description: 6 month |
anti-Separase |
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YF-PA25396 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to Separase |
LEXSYcon2.1 Expression Kit M-EGE-1310ble ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight |
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M-EGE-1310ble | MiTeGen | 1 Kit | EUR 1682 |
Description: LEXSYcon2.1 Expression Kit M-EGE-1310ble ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight |
Often resulting in prolonged mitotic apoptosis1. Abbreviated tumorigenic mitosis causing aneuploidy, but it is not clear whether it also activates apoptosis machinery2. Separase, cysteine proteases and triggers of all anaphases eukaryotic, have caspase-like catalytic domains but have never been associated with cell death3,4. Here we show that human cells entering mitosis with separase already active to quickly undergo mitosis death because cleavage directly MCL1 and anti-apoptotic BCL-XL by separase. Cleavage is not only prevent MCL1 and BCL-XL from the execution of pro-apoptotic BAK, but also convert them into active promoters of death in mitosis.
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