Tetraploidy, a common feature on cancer, results in the presence of extra centrosomes, which have been associated with chromosomal instability (CIN) and aneuploidy. Deregulation of the number of centrosomes trigger tumorigenesis. However, what supernumerary centrosomes evolved during the rise of tetraploid cells is still not explained.

Here, generate isogenic tetraploid clones in colorectal cancer and non-transformed cells, we showed that the tetraploid near-clones showed a significant increase in the number of centrosomes. In addition, we found that the centrosome area near-tetraploids is twice as large in the near-diploid. To evaluate whether centrosome clustering that happens, we analyzed the number of centrioles reveal amplification centrioles.

Although more than half of the near-tetraploids maintained in culture centrosome aberrations not present. To test whether the cells are increasingly lost centrioles after being near-tetraploid, we transiently transfected diploid cells with siRNA against ESPL1 / Separase, protease responsible for triggering anaphase, to generate new cells in near-tetraploid. Finally, using this model, we assess the number of centrioles at different time-points after tetraploidization found that near-tetraploids quickly centrosomes lose from time to time.

Taken together, these data indicate that although the majority of the cells reduces the supernumerary centrosomes after tetraploidization, a small percentage remained extra centrioles, potentially resulting in CIN.

Dun1, Chk2 kinase-related, is the central regulator of securin-separase dynamics during DNA damage signal.

DNA damage checkpoint stop cycle progression at G2 cells in response to genotoxic insults. Central to the implementation of cell cycle checkpoint arrest is induced stabilization securin-separase complex (yeast Pds1-Esp1). checkpoint kinase Chk1 and Chk2 (yeast Chk1 and Rad53) is expected to contribute to the stability of the critical-separase securin complex by phosphorylation securin, rendering it resistant to proteolytic breakdown by the anaphase promoting complex (APC).

Dun1, a Rad53 paralog associated with Chk2, are also important for the post-imposed arrest. Dun1 required for transcription-induced DNA damage DNA repair genes; However, its role in the implementation of cell cycle arrest is still unknown. Here, we show that Dun1 role in the arrest checkpoint independent of its involvement in the improvement of gene transcription.

Instead, Dun1 Pds1 necessary to prevent damage during DNA damage in cells Dun1-deficiency lowers Pds1, escape G2 arrest and undergoing mitotic checkpoint despite the presence of active Chk1 and Rad53. Interestingly, the proteolytic degradation of Pds1 the absence Dun1 mediated not by APC but by HECT domain containing E3 ligase Rsp5. Our results demonstrate regulatory scheme where Dun1 prevent chromosome segregation during DNA damage by inhibiting Rsp5-mediated proteolytic degradation of securin Pds1.

SEPARASE antibody
70R-31054	Fitzgerald	100 ug	EUR 392.4
Description: Rabbit polyclonal SEPARASE antibody

Separase Antibody
AF9195	Affbiotech	200ul	EUR 420

Separase antibody
70R-51689	Fitzgerald	100 ul	EUR 344.4
Description: Purified Polyclonal Separase antibody

Separase Antibody
ABF9195	Lifescience Market	100 ug	EUR 525.6

SEPARASE antibody (Ser801)
70R-31053	Fitzgerald	100 ug	EUR 392.4
Description: Rabbit polyclonal SEPARASE antibody (Ser801)

Separase (pS801) Antibody
20-abx134111	Abbexa	EUR 376.80 EUR 560.40 EUR 243.60	100 ul 200 ul 30 ul

Separase Conjugated Antibody
C44415	SAB	100ul	EUR 476.4

Separase Polyclonal Antibody
ABP52424-003ml	Abbkine	0.03ml	EUR 189.6
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody
ABP52424-01ml	Abbkine	0.1ml	EUR 346.8
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody
ABP52424-02ml	Abbkine	0.2ml	EUR 496.8
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody
ES3423-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase Polyclonal Antibody
ES3423-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Polyclonal ESPL1 / Separase Antibody (aa767-816)
APR02989G	Leading Biology	0.05ml	EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ESPL1 / Separase (aa767-816). This antibody is tested and proven to work in the following applications:

Separase (phospho Ser801) Polyclonal Antibody
ABP50519-003ml	Abbkine	0.03ml	EUR 189.6
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody
ABP50519-01ml	Abbkine	0.1ml	EUR 346.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody
ABP50519-02ml	Abbkine	0.2ml	EUR 496.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody
ES1518-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (phospho Ser801) Polyclonal Antibody
ES1518-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (Phospho-Ser801) Polyclonal Antibody
12353-100ul	SAB	100ul	EUR 302.4

Separase (Phospho-Ser801) Polyclonal Antibody
12353-50ul	SAB	50ul	EUR 224.4

Separase Blocking Peptide
AF9195-BP	Affbiotech	1mg	EUR 234

Separase Blocking Peptide
20-abx161616	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

SEPARASE Cell ELISA Kit
abx595539-96tests	Abbexa	96 tests	EUR 764.4

Separase (pS801) Blocking Peptide
20-abx162232	Abbexa	EUR 376.80 EUR 610.80	1 mg 5 mg

SEPARASE Colorimetric Cell-Based ELISA Kit
EKC1516	BosterBio	100ul	EUR 686.4

SEPARASE Colorimetric Cell-Based ELISA Kit (OKAG01028)
OKAG01028	Aviva Systems Biology	96 Wells	EUR 715.2
Description: Description of target: ;Species reactivity: Human, Mouse;Application: ELISA;Assay info: Assay Type: Cell-Based Subtype: None Detection Method: Colorimetric 450 nm;Sensitivity:

SEPARASE (Phospho-Ser801) Colorimetric Cell-Based ELISA Kit
EKC2000	BosterBio	100ul	EUR 686.4

Addi platform separators (4 ea) Add 1.25 to platform separation
MIX7118	Scientific Laboratory Supplies	EACH	EUR 74.4

Separator
INS6224	Scientific Laboratory Supplies	EACH	EUR 20.4

Additional platform separators (4 ea.). Adds 1.25" to platform separation
BR2000-SP	Benchmark Scientific	1 PC	EUR 52.8

Separin (ESPL1) Antibody
20-abx212982	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Separin (ESPL1) Antibody
20-abx211834	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Separin (ESPL1) Antibody
20-abx326179	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Separin (ESPL1) Antibody
20-abx301075	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Anti-Separase Antibody
A04364	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for Separase Antibody (ESPL1) detection. Tested with WB, IHC, IF in Human, Mouse.

Anti-Separase antibody
STJ95608	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to Separase.

Puck Separator
M-CBP-PST1	MiTeGen	1 UNIT	EUR 340
Description: Puck Separator

Separator UB 200mm
SIE1104	Scientific Laboratory Supplies	EACH	EUR 126.64

SEPARIN (pS1126) Antibody
abx031912-400ul	Abbexa	400 ul	EUR 627.6

SEPARIN (pS1126) Antibody
abx031912-80l	Abbexa	80 µl	EUR 343.2

Separin (ESPL1) Antibody (HRP)
20-abx316258	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Separin (ESPL1) Antibody (FITC)
20-abx316259	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Phase Separator 15ml
CHR5382	Scientific Laboratory Supplies	PK100	EUR 139.2

Phase Separator 25ml
CHR5384	Scientific Laboratory Supplies	PK100	EUR 152.76

Phase Separator 70ml
CHR5386	Scientific Laboratory Supplies	PK50	EUR 116.4

Phase Separator 150ml
CHR5388	Scientific Laboratory Supplies	PK25	EUR 145.2

Puck Separator Tools
M-CP-111-028	MiTeGen	1 UNIT	EUR 305
Description: Puck Separator Tools

Magnetic Separator
B23803	Bimake	2 mL/200 uL/15 mL	EUR 291.6
Description: This Magnetic Separator is a proprietary machine, supportinga the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time.

Magnetic Separator
MagSep1-1	ABTBeads	1/pk	EUR 132

McDonald Separator
INS6226	Scientific Laboratory Supplies	EACH	EUR 16.8

Separin (ESPL1) Antibody (Biotin)
20-abx316260	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

MiniTube Mag Separator
MTMS-16	Spherotech	mL	EUR 432
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Jenway Water Separator
FLA1034	Scientific Laboratory Supplies	EACH	EUR 184.8

Separating Funnel PP 500ml
FUN21060	Scientific Laboratory Supplies	PK2	EUR 93.6

50ml Separating Funnel
FUN4123	Scientific Laboratory Supplies	EACH	EUR 104.4

Pyrex 1L Separating Funnel
FUN4126	Scientific Laboratory Supplies	EACH	EUR 139.2

Pyrex 2L Separating Funnel
FUN4128	Scientific Laboratory Supplies	EACH	EUR 187.2

Separatory Funnel Pear 500ml
2394T/500	Scientific Laboratory Supplies	PK2	EUR 244.8

Magnetic Separation Rack
M1501-1	Biovision	1 Rack	EUR 454.8

Separating Funnel Pear 1000ml
FUN20650	Scientific Laboratory Supplies	PK2	EUR 205.2

Separating Funnel Pear 50ml
FUN2081	Scientific Laboratory Supplies	EACH	EUR 74.4

Separating Funnel Pear 100ml
FUN2083	Scientific Laboratory Supplies	EACH	EUR 85.2

Separating Funnel Pear 250ml
FUN2085	Scientific Laboratory Supplies	EACH	EUR 90

2L P/Shaped Separating Funnel
FUN4310	Scientific Laboratory Supplies	EACH	EUR 207.6

Separating Funnel Holder
STA1094	Scientific Laboratory Supplies	EACH	EUR 39.24

Magnetic Separation Rack
CM101	Vazyme	32 well (200 μl/well)	EUR 112

Magnetic Separation Rack
CM103	Vazyme	24 well (1.5 ml/well)	EUR 112

Separating funnel 100ML PTFE Key
8S149/100	Scientific Laboratory Supplies	EACH	EUR 45.6

Separating Funnel 500ml PTFE Key
8S149500	Scientific Laboratory Supplies	EACH	EUR 56.4

Jenway Water Separator Large
FLA1036	Scientific Laboratory Supplies	EACH	EUR 224.4

Pyrex 100ml Separating Funnel
FUN4120	Scientific Laboratory Supplies	EACH	EUR 104.4

Pyrex 250ml Separating Funnel
FUN4122	Scientific Laboratory Supplies	EACH	EUR 114

Pyrex 500ml Separating Funnel
FUN4124	Scientific Laboratory Supplies	EACH	EUR 120

Funnel Pyrex Separating Pear 5L
FUN4312	Scientific Laboratory Supplies	EACH	EUR 309.6

Cardboard Separator kit for xxxx
CRY2076	Scientific Laboratory Supplies	PK6	EUR 26.4

Long Form Separating Funnel 50ml
8S14950	Scientific Laboratory Supplies	EACH	EUR 44.4

50ml P/Shaped Separating Funnel
FUN4300	Scientific Laboratory Supplies	EACH	EUR 110.4

Magnetic Separator (Re-usable)
1999-1	Biovision	each	EUR 319.2

MicroMag Magnetic Separator
MMS-2100	Spherotech	mL	EUR 498
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

FlexiMag Magnetic Separator
FMS-1000	Spherotech	mL	EUR 583.2
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

FlexiMag Magnetic Separator
FMJ-1000	Spherotech	mL	EUR 432
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Nalgene 125ml Separating Funnel PP
FUN4180	Scientific Laboratory Supplies	EACH	EUR 97.2

HandiMag Magnetic Separator
HMS-1000	Spherotech	mL	EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

UltraMag DW Magnetic Separator
UMDS-1000	Spherotech	mL	EUR 741.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

UltraMag Magnetic Separator
UMS-3000	Spherotech	mL	EUR 736.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Statebourne 1 2 BSP Phase Separator
CRY2104	Scientific Laboratory Supplies	EACH	EUR 147.53

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA
WHA2200070	Scientific Laboratory Supplies	EACH	EUR 45.6

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA
WHA2200110	Scientific Laboratory Supplies	PK100	EUR 60

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA
WHA2200125	Scientific Laboratory Supplies	PK100	EUR 68.4

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA
WHA2200150	Scientific Laboratory Supplies	PK100	EUR 96

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA
WHA2200185	Scientific Laboratory Supplies	EACH	EUR 110.4

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA
WHA2200240	Scientific Laboratory Supplies	EACH	EUR 154.8

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA
WHA2200270	Scientific Laboratory Supplies	EACH	EUR 238.8

T-Pro Separating or Resolving Buffer
JB03-C001	T-Pro Biotechnology	500ml/BT	EUR 182.4

EpiMag HT (96-Well) Magnetic Separator
Q10002	EpiGentek	EUR 416.70 EUR 258.50	1/Pack 1/Pack

WHATMAN(R) TG100 SEPARATION GAUZE DIAM
WHA10423029	Scientific Laboratory Supplies	EACH	EUR 174

100 ML LSM (LYMPHOCYTE SEPARATION MEDIUM)
25-072-CI	CORNING	100 mL/pk	EUR 63.6
Description: Media Catalog; Cell Culture Reagents

Separator type 10001 for drawers capacity 10000 cm3
DD039473	Scientific Laboratory Supplies	EACH	EUR 7.2

PCR 8-Strips, 0.2ml with Separate Flat Cap Strips, 120/pk
P3010-S	MTC Bio	120/pack	EUR 116.7
Description: PCR 8-Strips 0.2ml with Separate Flat Cap Strips

Culture Tube, 5mL, 12 x 75mm, PP, w/ separate 2-position screw-cap, non-sterile, non-graduated, 1000 tubes and capped bulk packed separately
T8750	MTC Bio	1000/pack	EUR 147.48
Description: Culture Tube 5mL

Culture Tube, 14mL, 17 x 100mm, PP, w/ separate 2-position screw-cap, non-sterile, non-graduated, 1000 tubes and capped bulk packed separately
T8850	MTC Bio	1000/pack	EUR 228.48
Description: Culture Tube 14mL

Lymphocyte separation medium (human)
abx082102-200ml	Abbexa	200 ml	EUR 243.6

Cytosol/Particulate Separation Kit
55R-1356	Fitzgerald	50 assays	EUR 661.2
Description: Cytosol/Particulate Separation Kit for use in the research laboratory

Cytosol/Particulate Separation Kit
K267-50	Biovision	each	EUR 483.6

Cytosol/Particulate Separation Kit
K2112-50	ApexBio	50 assays	EUR 501.6

WHATMAN 1PS PHASE SEPARATOR FILTER PAPER
WHA2200090	Scientific Laboratory Supplies	EACH	EUR 49.2

1-Step Polymorphs, Human Cell Separation
71-AN221725	Gentaur Genprice	1	EUR 238.8

Taq DNA Polymerase - 5,000 units with separate dNTP Mix
3245d	Intact Genomics	1/EA	EUR 802.8

Separin Phospho-Ser801 (ESPL1 pS801) Antibody
20-abx326180	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Techne Separation Membranes 200x200mm
OVE3038	Scientific Laboratory Supplies	PK5	EUR 181.2

Custom drilling for BSW01 (sold separately) up to 36 holes
BLO1274	Scientific Laboratory Supplies	EACH	EUR 260.4

Custom drilling for BSW01 (sold separately), up to 36 holes
BSW01-DR	Benchmark Scientific	1 PC	EUR 195.3

Funnel Separating Conical Ptfe Stopcock 100ml
E4606	Scientific Laboratory Supplies	EACH	EUR 33.6

Funnel Separating Conical Ptfe Stopcock 250ml
E4607	Scientific Laboratory Supplies	EACH	EUR 43.2

Orion Star A211 pH Benchtop Meter Separate Sensor Kit
STARA2118	Scientific Laboratory Supplies	EACH	EUR 1358.4

Mercury is supplied as a separate solution (PLHG-1Y).
TCLP-100	Scientific Laboratory Supplies	125ML	EUR 253.2

Mercury is supplied as a separate solution (PLHG-1X).
TCLP-500	Scientific Laboratory Supplies	500ML	EUR 429.6

Stacking platform, small 10.5"x7.5" with flat mat (3.0"separation)
B3D-STACK	Benchmark Scientific	1 PC	EUR 135.8

Corning 500mL LSM (Lymphocyte Separation Medium)
25-072-CV	Scientific Laboratory Supplies	EACH	EUR 156

Clear Glass Powder Bottle with Separate Black Cap - 15mL
BOT1200	Scientific Laboratory Supplies	PK140	EUR 87.6

Clear Glass Powder Bottle with Separate Black Cap - 30mL
BOT1201	Scientific Laboratory Supplies	PK80	EUR 49.2

Clear Glass Powder Bottle with Separate Black Cap - 60mL
BOT1204	Scientific Laboratory Supplies	PK72	EUR 50.4

Clear Glass Powder Bottle with Separate Black Cap - 125mL
BOT1206	Scientific Laboratory Supplies	PK80	EUR 60

Clear Glass Powder Bottle with Separate Black Cap - 250mL
BOT1211	Scientific Laboratory Supplies	PK42	EUR 37.2

Clear Glass Powder Bottle with Separate Black Cap - 500mL
BOT1212	Scientific Laboratory Supplies	PK33	EUR 50.4

Amber Glass Powder Bottle with Separate Black Cap - 30mL
BOT1220	Scientific Laboratory Supplies	PK127	EUR 81.6

Amber Glass Powder Bottle with Separate Black Cap - 60mL
BOT1222	Scientific Laboratory Supplies	PK72	EUR 40.8

Amber Glass Powder Bottle with Separate Black Cap - 250mL
BOT1226	Scientific Laboratory Supplies	PK42	EUR 74.4

Amber Glass Powder Bottle with Separate Black Cap - 500mL
BOT1228	Scientific Laboratory Supplies	PK28	EUR 39.6

Mercury is supplied as a separate solution (PLHG-1AX).
EP-8-500	Scientific Laboratory Supplies	500ML	EUR 366

Mercury is supplied as a separate solution (PLHG2-1Y).
LPC-1-100	Scientific Laboratory Supplies	125ML	EUR 528

Mercury is supplied as a separate solution (PLHG2-1X).
LPC-1-500	Scientific Laboratory Supplies	500ML	EUR 912

0.1ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), WHITE 120/pk
P3801-QW	MTC Bio	120/pack	EUR 162.06
Description: 0.1ml qPCR 8-Strip ,white 120

0.2ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), WHITE, 120/pk
P3802-QW	MTC Bio	120/pack	EUR 162.06
Description: 0.2ml qPCR 8-Strip , white

Stacking platform small 10.5x7.5 w flat mat (3.0separation)
MIX7112	Scientific Laboratory Supplies	EACH	EUR 182.4

Simax Separating Funnel Cylindrical Open 50ml
FUN2060	Scientific Laboratory Supplies	EACH	EUR 46.8

Simax Separating Funnel Cylindrical Open 100ml
FUN20660	Scientific Laboratory Supplies	PK4	EUR 180

Pyrex Separating Funnel 100ml Glass Stopcock Pear
FUN4322	Scientific Laboratory Supplies	EACH	EUR 117.6

Mercury is supplied as a separate solution (PLHG2-1Y).
INTER18-100	Scientific Laboratory Supplies	125ML	EUR 501.6

Mercury is supplied as a separate solution (PLHG2-1X).
INTER18-500	Scientific Laboratory Supplies	500ML	EUR 874.8

Mercury is supplied as a separate solution (PLHG2-AY).
WP-15	Scientific Laboratory Supplies	125ML	EUR 328.8

Stacking platform, large 12"x12" with flat mat (3.0" separation)
BR1000-STACK	Benchmark Scientific	1 PC	EUR 135.8

Mercury is supplied as a separate solution (PLHG2-1AY).
EP-8	Scientific Laboratory Supplies	125ML	EUR 220.8

PCR Tubes Strips of 8 tubes w/ dome caps packaged separately, 120/pk
P3020	MTC Bio	120/pack	EUR 118.32
Description: PCR Tubes Strips of 8 tubes w/ dome caps packaged separately120

Stacking platform large 12x12 w flat mat (3.0 separation)
MIX7114	Scientific Laboratory Supplies	EACH	EUR 182.4

Mercury is supplied as a separate solution (PLHG2-1AX).
WP-15-500	Scientific Laboratory Supplies	500ML	EUR 550.8

0.1ml qPCR 8-Strip (With SEPARATE Optical Strip Caps) FROSTED, 120/pk
P3801-QF	MTC Bio	120/pack	EUR 157.2
Description: 0.1ml qPCR 8-Strip , frosted, 120

Polar/Neutral Lipid Separation Kit (Chloroform Free)
MET-5009	Cell Biolabs	50 preps	EUR 428.4
Description: Our Polar/Neutral Lipid Separation Kit separates polar from neutral lipids starting from a total organic lipid pool obtained through extraction by the Folch method or our Lipid Extraction Kit. Polar and neutral lipid separation occurs through the addition of a proprietary secondary alcohol and a proprietary secondary organic compound. After phase separation, the top layer containing the neutral lipids is separated from polar lipids in the bottom layer. The layers are dried and the lipidsresuspended for downstream analysis.

Stacking platform, small 10.5"x7.5" with dimpled mat (3.0" separation)
B3D-STACK-D	Benchmark Scientific	1 PC	EUR 141

5mL Tubes, non-sterile, w/ screw caps packed separately, pk/500 ( 5 bags of 100)
C1005-SC5	Benchmark Scientific	1 each	EUR 99

Pyrex Morbank Separating Funnel 1L Glass Stopcock Pear
FUN4328	Scientific Laboratory Supplies	EACH	EUR 148.8

Anti-Phospho-Separase (S801) antibody
STJ91032	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to Phospho-Separase (S801).

Stacking platform, large 12"x12" with dimpled mat (3.0" separation)
BR1000-STACK-D	Benchmark Scientific	1 PC	EUR 141

Stacking Platform, extra large 14"x12" with flat mat (3.0" separation)
BR2000-STACK	Benchmark Scientific	1 PC	EUR 147.7

OrgFrontier? Viable/Non-Viable Cells Separation Kit
K850-10	Biovision	each	EUR 652.8

Stacking platform large 12x12 w dimpled mat (3.0 separation)
MIX7116	Scientific Laboratory Supplies	EACH	EUR 188.4

0.1ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), Natural/Clear, 120/pk
P3801-Q	MTC Bio	120/pack	EUR 157.2
Description: 0.1ml qPCR 8-Strip , Natural/Clear

0.2ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), Natural/Clear, 120/pk
P3802-Q	MTC Bio	120/pack	EUR 157.2
Description: 0.2ml qPCR 8-Strip , Natural/Clear

Pyrex Pear Shaped Separating Funnel with PTFE Stopcock 250ml
FUN4324	Scientific Laboratory Supplies	EACH	EUR 122.4

Custom drilling for BCM1412-BSW01 (sold separately), up to 36 holes
BCM1412-BSW01-DR	Bio Basic	1 pcs, 1 UNIT	EUR 289.42

Brand squibb separating funnel, pp stopper, ungraduated, ptfe key, 250 ml
Z330930-2EA	Scientific Laboratory Supplies	PK2	EUR 165.81

5mL Screw-Cap MacroTube, non-sterile, w/ screw caps packed separately
C2530	MTC Bio	500/pack	EUR 139.38
Description: 5mL MacroTubes

Shaking Platform Pre-drilled For Flask Clamps (clamps Sold Separately)
480159	Scientific Laboratory Supplies	EACH	EUR 430.8

Grant-Bio Orbital Shaker for Use in CO2 Incubators - Platforms Sold Separately
SHA7830	Scientific Laboratory Supplies	EACH	EUR 1984.8

Grant-Bio Overhead Stirrer Multi Mixer - Attachments Sold Separately
STI1270	Scientific Laboratory Supplies	EACH	EUR 732

Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free)
MET-5009-C	Cell Biolabs	50 preps	EUR 790.8
Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis.

LEXSY Cultivation Kit P10 ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight
M-LT-102	MiTeGen	1 Kit	EUR 1279
Description: LEXSY Cultivation Kit P10 ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

LEXSY Cultivation Kit T7-TR ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight
M-LT-111	MiTeGen	1 Kit	EUR 2841
Description: LEXSY Cultivation Kit T7-TR ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

TARGATT ? Transgenic Kit (5 microinjections) Academic Price *Plasmid sold separately
AST-1003	Applied StemCell	1 Kit (5 microinjections)	EUR 691.8
Description: 6 month

TARGATT ? Transgenic Kit (2 microinjections) Academic Price *Plasmid sold separately
AST-1004	Applied StemCell	1 Kit (2 microinjections)	EUR 388.06
Description: 6 month

anti-Separase
YF-PA25396	Abfrontier	50 ul	EUR 400.8
Description: Mouse polyclonal to Separase

LEXSYcon2.1 Expression Kit M-EGE-1310ble ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight
M-EGE-1310ble	MiTeGen	1 Kit	EUR 1682
Description: LEXSYcon2.1 Expression Kit M-EGE-1310ble ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

Often resulting in prolonged mitotic apoptosis1. Abbreviated tumorigenic mitosis causing aneuploidy, but it is not clear whether it also activates apoptosis machinery2. Separase, cysteine ​​proteases and triggers of all anaphases eukaryotic, have caspase-like catalytic domains but have never been associated with cell death3,4. Here we show that human cells entering mitosis with separase already active to quickly undergo mitosis death because cleavage directly MCL1 and anti-apoptotic BCL-XL by separase. Cleavage is not only prevent MCL1 and BCL-XL from the execution of pro-apoptotic BAK, but also convert them into active promoters of death in mitosis.