Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion

Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion

Tetraploidy, a common feature on cancer, results in the presence of extra centrosomes, which have been associated with chromosomal instability (CIN) and aneuploidy. Deregulation of the number of centrosomes trigger tumorigenesis. However, what supernumerary centrosomes evolved during the rise of tetraploid cells is still not explained.

Here, generate isogenic tetraploid clones in colorectal cancer and non-transformed cells, we showed that the tetraploid near-clones showed a significant increase in the number of centrosomes. In addition, we found that the centrosome area near-tetraploids is twice as large in the near-diploid. To evaluate whether centrosome clustering that happens, we analyzed the number of centrioles reveal amplification centrioles.

Although more than half of the near-tetraploids maintained in culture centrosome aberrations not present. To test whether the cells are increasingly lost centrioles after being near-tetraploid, we transiently transfected diploid cells with siRNA against ESPL1 / Separase, protease responsible for triggering anaphase, to generate new cells in near-tetraploid. Finally, using this model, we assess the number of centrioles at different time-points after tetraploidization found that near-tetraploids quickly centrosomes lose from time to time.

Taken together, these data indicate that although the majority of the cells reduces the supernumerary centrosomes after tetraploidization, a small percentage remained extra centrioles, potentially resulting in CIN.

Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion
Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion

Dun1, Chk2 kinase-related, is the central regulator of securin-separase dynamics during DNA damage signal.

DNA damage checkpoint stop cycle progression at G2 cells in response to genotoxic insults. Central to the implementation of cell cycle checkpoint arrest is induced stabilization securin-separase complex (yeast Pds1-Esp1). checkpoint kinase Chk1 and Chk2 (yeast Chk1 and Rad53) is expected to contribute to the stability of the critical-separase securin complex by phosphorylation securin, rendering it resistant to proteolytic breakdown by the anaphase promoting complex (APC).

Dun1, a Rad53 paralog associated with Chk2, are also important for the post-imposed arrest. Dun1 required for transcription-induced DNA damage DNA repair genes; However, its role in the implementation of cell cycle arrest is still unknown. Here, we show that Dun1 role in the arrest checkpoint independent of its involvement in the improvement of gene transcription.

Instead, Dun1 Pds1 necessary to prevent damage during DNA damage in cells Dun1-deficiency lowers Pds1, escape G2 arrest and undergoing mitotic checkpoint despite the presence of active Chk1 and Rad53. Interestingly, the proteolytic degradation of Pds1 the absence Dun1 mediated not by APC but by HECT domain containing E3 ligase Rsp5. Our results demonstrate regulatory scheme where Dun1 prevent chromosome segregation during DNA damage by inhibiting Rsp5-mediated proteolytic degradation of securin Pds1.

SEPARASE antibody

70R-31054 100 ug
EUR 392.4
Description: Rabbit polyclonal SEPARASE antibody

Separase Antibody

AF9195 200ul
EUR 420

Separase antibody

70R-51689 100 ul
EUR 344.4
Description: Purified Polyclonal Separase antibody

Separase Antibody

ABF9195 100 ug
EUR 525.6

SEPARASE antibody (Ser801)

70R-31053 100 ug
EUR 392.4
Description: Rabbit polyclonal SEPARASE antibody (Ser801)

Separase (pS801) Antibody

20-abx134111
  • EUR 376.80
  • EUR 560.40
  • EUR 243.60
  • 100 ul
  • 200 ul
  • 30 ul

Separase Conjugated Antibody

C44415 100ul
EUR 476.4

Separase Polyclonal Antibody

ABP52424-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ABP52424-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ABP52424-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ES3423-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase Polyclonal Antibody

ES3423-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Polyclonal ESPL1 / Separase Antibody (aa767-816)

APR02989G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ESPL1 / Separase (aa767-816). This antibody is tested and proven to work in the following applications:

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ES1518-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (phospho Ser801) Polyclonal Antibody

ES1518-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (Phospho-Ser801) Polyclonal Antibody

12353-100ul 100ul
EUR 302.4

Separase (Phospho-Ser801) Polyclonal Antibody

12353-50ul 50ul
EUR 224.4

Separase Blocking Peptide

AF9195-BP 1mg
EUR 234

Separase Blocking Peptide

20-abx161616
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

SEPARASE Cell ELISA Kit

abx595539-96tests 96 tests
EUR 764.4

Separase (pS801) Blocking Peptide

20-abx162232
  • EUR 376.80
  • EUR 610.80
  • 1 mg
  • 5 mg

SEPARASE Colorimetric Cell-Based ELISA Kit

EKC1516 100ul
EUR 686.4

SEPARASE Colorimetric Cell-Based ELISA Kit (OKAG01028)

OKAG01028 96 Wells
EUR 715.2
Description: Description of target: ;Species reactivity: Human, Mouse;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:

SEPARASE (Phospho-Ser801) Colorimetric Cell-Based ELISA Kit

EKC2000 100ul
EUR 686.4

Addi platform separators (4 ea) Add 1.25 to platform separation

MIX7118 EACH
EUR 74.4

Separator

INS6224 EACH
EUR 20.4

Additional platform separators (4 ea.). Adds 1.25" to platform separation

BR2000-SP 1 PC
EUR 52.8

Separin (ESPL1) Antibody

20-abx212982
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Separin (ESPL1) Antibody

20-abx211834
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Separin (ESPL1) Antibody

20-abx326179
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Separin (ESPL1) Antibody

20-abx301075
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Separase Antibody

A04364 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Separase Antibody (ESPL1) detection. Tested with WB, IHC, IF in Human, Mouse.

Anti-Separase antibody

STJ95608 200 µl
EUR 236.4
Description: Rabbit polyclonal to Separase.

Puck Separator

M-CBP-PST1 1 UNIT
EUR 340
Description: Puck Separator

Separator UB 200mm

SIE1104 EACH
EUR 126.64

SEPARIN (pS1126) Antibody

abx031912-400ul 400 ul
EUR 627.6

SEPARIN (pS1126) Antibody

abx031912-80l 80 µl
EUR 343.2

Separin (ESPL1) Antibody (HRP)

20-abx316258
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Separin (ESPL1) Antibody (FITC)

20-abx316259
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Phase Separator 15ml

CHR5382 PK100
EUR 139.2

Phase Separator 25ml

CHR5384 PK100
EUR 152.76

Phase Separator 70ml

CHR5386 PK50
EUR 116.4

Phase Separator 150ml

CHR5388 PK25
EUR 145.2

Puck Separator Tools

M-CP-111-028 1 UNIT
EUR 305
Description: Puck Separator Tools

Magnetic Separator

B23803 2 mL/200 uL/15 mL
EUR 291.6
Description: This Magnetic Separator is a proprietary machine, supportinga the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time.

Magnetic Separator

MagSep1-1 1/pk
EUR 132

McDonald Separator

INS6226 EACH
EUR 16.8

Separin (ESPL1) Antibody (Biotin)

20-abx316260
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

MiniTube Mag Separator

MTMS-16 mL
EUR 432
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Jenway Water Separator

FLA1034 EACH
EUR 184.8

Separating Funnel PP 500ml

FUN21060 PK2
EUR 93.6

50ml Separating Funnel

FUN4123 EACH
EUR 104.4

Pyrex 1L Separating Funnel

FUN4126 EACH
EUR 139.2

Pyrex 2L Separating Funnel

FUN4128 EACH
EUR 187.2

Separatory Funnel Pear 500ml

2394T/500 PK2
EUR 244.8

Magnetic Separation Rack

M1501-1 1 Rack
EUR 454.8

Separating Funnel Pear 1000ml

FUN20650 PK2
EUR 205.2

Separating Funnel Pear 50ml

FUN2081 EACH
EUR 74.4

Separating Funnel Pear 100ml

FUN2083 EACH
EUR 85.2

Separating Funnel Pear 250ml

FUN2085 EACH
EUR 90

2L P/Shaped Separating Funnel

FUN4310 EACH
EUR 207.6

Separating Funnel Holder

STA1094 EACH
EUR 39.24

Magnetic Separation Rack

CM101 32 well (200 μl/well)
EUR 112

Magnetic Separation Rack

CM103 24 well (1.5 ml/well)
EUR 112

Separating funnel 100ML PTFE Key

8S149/100 EACH
EUR 45.6

Separating Funnel 500ml PTFE Key

8S149500 EACH
EUR 56.4

Jenway Water Separator Large

FLA1036 EACH
EUR 224.4

Pyrex 100ml Separating Funnel

FUN4120 EACH
EUR 104.4

Pyrex 250ml Separating Funnel

FUN4122 EACH
EUR 114

Pyrex 500ml Separating Funnel

FUN4124 EACH
EUR 120

Funnel Pyrex Separating Pear 5L

FUN4312 EACH
EUR 309.6

Cardboard Separator kit for xxxx

CRY2076 PK6
EUR 26.4

Long Form Separating Funnel 50ml

8S14950 EACH
EUR 44.4

50ml P/Shaped Separating Funnel

FUN4300 EACH
EUR 110.4

Magnetic Separator (Re-usable)

1999-1 each
EUR 319.2

MicroMag Magnetic Separator

MMS-2100 mL
EUR 498
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

FlexiMag Magnetic Separator

FMS-1000 mL
EUR 583.2
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

FlexiMag Magnetic Separator

FMJ-1000 mL
EUR 432
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Nalgene 125ml Separating Funnel PP

FUN4180 EACH
EUR 97.2

HandiMag Magnetic Separator

HMS-1000 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

UltraMag DW Magnetic Separator

UMDS-1000 mL
EUR 741.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

UltraMag Magnetic Separator

UMS-3000 mL
EUR 736.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Statebourne 1 2 BSP Phase Separator

CRY2104 EACH
EUR 147.53

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200070 EACH
EUR 45.6

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200110 PK100
EUR 60

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200125 PK100
EUR 68.4

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200150 PK100
EUR 96

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200185 EACH
EUR 110.4

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200240 EACH
EUR 154.8

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200270 EACH
EUR 238.8

T-Pro Separating or Resolving Buffer

JB03-C001 500ml/BT
EUR 182.4

EpiMag HT (96-Well) Magnetic Separator

Q10002
  • EUR 416.70
  • EUR 258.50
  • 1/Pack
  • 1/Pack

WHATMAN(R) TG100 SEPARATION GAUZE DIAM

WHA10423029 EACH
EUR 174

100 ML LSM (LYMPHOCYTE SEPARATION MEDIUM)

25-072-CI 100 mL/pk
EUR 63.6
Description: Media Catalog; Cell Culture Reagents

Separator type 10001 for drawers capacity 10000 cm3

DD039473 EACH
EUR 7.2

PCR 8-Strips, 0.2ml with Separate Flat Cap Strips, 120/pk

P3010-S 120/pack
EUR 116.7
Description: PCR 8-Strips 0.2ml with Separate Flat Cap Strips

Culture Tube, 5mL, 12 x 75mm, PP, w/ separate 2-position screw-cap, non-sterile, non-graduated, 1000 tubes and capped bulk packed separately

T8750 1000/pack
EUR 147.48
Description: Culture Tube 5mL

Culture Tube, 14mL, 17 x 100mm, PP, w/ separate 2-position screw-cap, non-sterile, non-graduated, 1000 tubes and capped bulk packed separately

T8850 1000/pack
EUR 228.48
Description: Culture Tube 14mL

Lymphocyte separation medium (human)

abx082102-200ml 200 ml
EUR 243.6

Cytosol/Particulate Separation Kit

55R-1356 50 assays
EUR 661.2
Description: Cytosol/Particulate Separation Kit for use in the research laboratory

Cytosol/Particulate Separation Kit

K267-50 each
EUR 483.6

Cytosol/Particulate Separation Kit

K2112-50 50 assays
EUR 501.6

WHATMAN 1PS PHASE SEPARATOR FILTER PAPER

WHA2200090 EACH
EUR 49.2

1-Step Polymorphs, Human Cell Separation

71-AN221725 1
EUR 238.8

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 802.8

Separin Phospho-Ser801 (ESPL1 pS801) Antibody

20-abx326180
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Techne Separation Membranes 200x200mm

OVE3038 PK5
EUR 181.2

Custom drilling for BSW01 (sold separately) up to 36 holes

BLO1274 EACH
EUR 260.4

Custom drilling for BSW01 (sold separately), up to 36 holes

BSW01-DR 1 PC
EUR 195.3

Funnel Separating Conical Ptfe Stopcock 100ml

E4606 EACH
EUR 33.6

Funnel Separating Conical Ptfe Stopcock 250ml

E4607 EACH
EUR 43.2

Orion Star A211 pH Benchtop Meter Separate Sensor Kit

STARA2118 EACH
EUR 1358.4

Mercury is supplied as a separate solution (PLHG-1Y).

TCLP-100 125ML
EUR 253.2

Mercury is supplied as a separate solution (PLHG-1X).

TCLP-500 500ML
EUR 429.6

Stacking platform, small 10.5"x7.5" with flat mat (3.0"separation)

B3D-STACK 1 PC
EUR 135.8

Corning 500mL LSM (Lymphocyte Separation Medium)

25-072-CV EACH
EUR 156

Clear Glass Powder Bottle with Separate Black Cap - 15mL

BOT1200 PK140
EUR 87.6

Clear Glass Powder Bottle with Separate Black Cap - 30mL

BOT1201 PK80
EUR 49.2

Clear Glass Powder Bottle with Separate Black Cap - 60mL

BOT1204 PK72
EUR 50.4

Clear Glass Powder Bottle with Separate Black Cap - 125mL

BOT1206 PK80
EUR 60

Clear Glass Powder Bottle with Separate Black Cap - 250mL

BOT1211 PK42
EUR 37.2

Clear Glass Powder Bottle with Separate Black Cap - 500mL

BOT1212 PK33
EUR 50.4

Amber Glass Powder Bottle with Separate Black Cap - 30mL

BOT1220 PK127
EUR 81.6

Amber Glass Powder Bottle with Separate Black Cap - 60mL

BOT1222 PK72
EUR 40.8

Amber Glass Powder Bottle with Separate Black Cap - 250mL

BOT1226 PK42
EUR 74.4

Amber Glass Powder Bottle with Separate Black Cap - 500mL

BOT1228 PK28
EUR 39.6

Mercury is supplied as a separate solution (PLHG-1AX).

EP-8-500 500ML
EUR 366

Mercury is supplied as a separate solution (PLHG2-1Y).

LPC-1-100 125ML
EUR 528

Mercury is supplied as a separate solution (PLHG2-1X).

LPC-1-500 500ML
EUR 912

0.1ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), WHITE 120/pk

P3801-QW 120/pack
EUR 162.06
Description: 0.1ml qPCR 8-Strip ,white 120

0.2ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), WHITE, 120/pk

P3802-QW 120/pack
EUR 162.06
Description: 0.2ml qPCR 8-Strip , white

Stacking platform small 10.5x7.5 w flat mat (3.0separation)

MIX7112 EACH
EUR 182.4

Simax Separating Funnel Cylindrical Open 50ml

FUN2060 EACH
EUR 46.8

Simax Separating Funnel Cylindrical Open 100ml

FUN20660 PK4
EUR 180

Pyrex Separating Funnel 100ml Glass Stopcock Pear

FUN4322 EACH
EUR 117.6

Mercury is supplied as a separate solution (PLHG2-1Y).

INTER18-100 125ML
EUR 501.6

Mercury is supplied as a separate solution (PLHG2-1X).

INTER18-500 500ML
EUR 874.8

Mercury is supplied as a separate solution (PLHG2-AY).

WP-15 125ML
EUR 328.8

Stacking platform, large 12"x12" with flat mat (3.0" separation)

BR1000-STACK 1 PC
EUR 135.8

Mercury is supplied as a separate solution (PLHG2-1AY).

EP-8 125ML
EUR 220.8

PCR Tubes Strips of 8 tubes w/ dome caps packaged separately, 120/pk

P3020 120/pack
EUR 118.32
Description: PCR Tubes Strips of 8 tubes w/ dome caps packaged separately120

Stacking platform large 12x12 w flat mat (3.0 separation)

MIX7114 EACH
EUR 182.4

Mercury is supplied as a separate solution (PLHG2-1AX).

WP-15-500 500ML
EUR 550.8

0.1ml qPCR 8-Strip (With SEPARATE Optical Strip Caps) FROSTED, 120/pk

P3801-QF 120/pack
EUR 157.2
Description: 0.1ml qPCR 8-Strip , frosted, 120

Polar/Neutral Lipid Separation Kit (Chloroform Free)

MET-5009 50 preps
EUR 428.4
Description: Our Polar/Neutral Lipid Separation Kit separates polar from neutral lipids starting from a total organic lipid pool obtained through extraction by the Folch method or our Lipid Extraction Kit. Polar and neutral lipid separation occurs through the addition of a proprietary secondary alcohol and a proprietary secondary organic compound. After phase separation, the top layer containing the neutral lipids is separated from polar lipids in the bottom layer. The layers are dried and the lipidsresuspended for downstream analysis.

Stacking platform, small 10.5"x7.5" with dimpled mat (3.0" separation)

B3D-STACK-D 1 PC
EUR 141

5mL Tubes, non-sterile, w/ screw caps packed separately, pk/500 ( 5 bags of 100)

C1005-SC5 1 each
EUR 99

Pyrex Morbank Separating Funnel 1L Glass Stopcock Pear

FUN4328 EACH
EUR 148.8

Anti-Phospho-Separase (S801) antibody

STJ91032 200 µl
EUR 236.4
Description: Rabbit polyclonal to Phospho-Separase (S801).

Stacking platform, large 12"x12" with dimpled mat (3.0" separation)

BR1000-STACK-D 1 PC
EUR 141

Stacking Platform, extra large 14"x12" with flat mat (3.0" separation)

BR2000-STACK 1 PC
EUR 147.7

OrgFrontier? Viable/Non-Viable Cells Separation Kit

K850-10 each
EUR 652.8

Stacking platform large 12x12 w dimpled mat (3.0 separation)

MIX7116 EACH
EUR 188.4

0.1ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), Natural/Clear, 120/pk

P3801-Q 120/pack
EUR 157.2
Description: 0.1ml qPCR 8-Strip , Natural/Clear

0.2ml qPCR 8-Strip (With SEPARATE Optical Strip Caps), Natural/Clear, 120/pk

P3802-Q 120/pack
EUR 157.2
Description: 0.2ml qPCR 8-Strip , Natural/Clear

Pyrex Pear Shaped Separating Funnel with PTFE Stopcock 250ml

FUN4324 EACH
EUR 122.4

Custom drilling for BCM1412-BSW01 (sold separately), up to 36 holes

BCM1412-BSW01-DR 1 pcs, 1 UNIT
EUR 289.42

Brand squibb separating funnel, pp stopper, ungraduated, ptfe key, 250 ml

Z330930-2EA PK2
EUR 165.81

5mL Screw-Cap MacroTube, non-sterile, w/ screw caps packed separately

C2530 500/pack
EUR 139.38
Description: 5mL MacroTubes

Shaking Platform Pre-drilled For Flask Clamps (clamps Sold Separately)

480159 EACH
EUR 430.8

Grant-Bio Orbital Shaker for Use in CO2 Incubators - Platforms Sold Separately

SHA7830 EACH
EUR 1984.8

Grant-Bio Overhead Stirrer Multi Mixer - Attachments Sold Separately

STI1270 EACH
EUR 732

Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free)

MET-5009-C 50 preps
EUR 790.8
Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis.

LEXSY Cultivation Kit P10 ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

M-LT-102 1 Kit
EUR 1279
Description: LEXSY Cultivation Kit P10 ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

LEXSY Cultivation Kit T7-TR ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

M-LT-111 1 Kit
EUR 2841
Description: LEXSY Cultivation Kit T7-TR ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

TARGATT ? Transgenic Kit (5 microinjections) Academic Price *Plasmid sold separately

AST-1003 1 Kit (5 microinjections)
EUR 691.8
Description: 6 month

TARGATT ? Transgenic Kit (2 microinjections) Academic Price *Plasmid sold separately

AST-1004 1 Kit (2 microinjections)
EUR 388.06
Description: 6 month

anti-Separase

YF-PA25396 50 ul
EUR 400.8
Description: Mouse polyclonal to Separase

LEXSYcon2.1 Expression Kit M-EGE-1310ble ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

M-EGE-1310ble 1 Kit
EUR 1682
Description: LEXSYcon2.1 Expression Kit M-EGE-1310ble ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight


Often resulting in prolonged mitotic apoptosis1. Abbreviated tumorigenic mitosis causing aneuploidy, but it is not clear whether it also activates apoptosis machinery2. Separase, cysteine ​​proteases and triggers of all anaphases eukaryotic, have caspase-like catalytic domains but have never been associated with cell death3,4. Here we show that human cells entering mitosis with separase already active to quickly undergo mitosis death because cleavage directly MCL1 and anti-apoptotic BCL-XL by separase. Cleavage is not only prevent MCL1 and BCL-XL from the execution of pro-apoptotic BAK, but also convert them into active promoters of death in mitosis.

Karina

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