Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion

Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion

Tetraploidy, a common feature on cancer, results in the presence of extra centrosomes, which have been associated with chromosomal instability (CIN) and aneuploidy. Deregulation of the number of centrosomes trigger tumorigenesis. However, what supernumerary centrosomes evolved during the rise of tetraploid cells is still not explained.

Here, generate isogenic tetraploid clones in colorectal cancer and non-transformed cells, we showed that the tetraploid near-clones showed a significant increase in the number of centrosomes. In addition, we found that the centrosome area near-tetraploids is twice as large in the near-diploid. To evaluate whether centrosome clustering that happens, we analyzed the number of centrioles reveal amplification centrioles.

Although more than half of the near-tetraploids maintained in culture centrosome aberrations not present. To test whether the cells are increasingly lost centrioles after being near-tetraploid, we transiently transfected diploid cells with siRNA against ESPL1 / Separase, protease responsible for triggering anaphase, to generate new cells in near-tetraploid. Finally, using this model, we assess the number of centrioles at different time-points after tetraploidization found that near-tetraploids quickly centrosomes lose from time to time.

Taken together, these data indicate that although the majority of the cells reduces the supernumerary centrosomes after tetraploidization, a small percentage remained extra centrioles, potentially resulting in CIN.

Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion
Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion

Dun1, Chk2 kinase-related, is the central regulator of securin-separase dynamics during DNA damage signal.

DNA damage checkpoint stop cycle progression at G2 cells in response to genotoxic insults. Central to the implementation of cell cycle checkpoint arrest is induced stabilization securin-separase complex (yeast Pds1-Esp1). checkpoint kinase Chk1 and Chk2 (yeast Chk1 and Rad53) is expected to contribute to the stability of the critical-separase securin complex by phosphorylation securin, rendering it resistant to proteolytic breakdown by the anaphase promoting complex (APC).

Dun1, a Rad53 paralog associated with Chk2, are also important for the post-imposed arrest. Dun1 required for transcription-induced DNA damage DNA repair genes; However, its role in the implementation of cell cycle arrest is still unknown. Here, we show that Dun1 role in the arrest checkpoint independent of its involvement in the improvement of gene transcription.

Instead, Dun1 Pds1 necessary to prevent damage during DNA damage in cells Dun1-deficiency lowers Pds1, escape G2 arrest and undergoing mitotic checkpoint despite the presence of active Chk1 and Rad53. Interestingly, the proteolytic degradation of Pds1 the absence Dun1 mediated not by APC but by HECT domain containing E3 ligase Rsp5. Our results demonstrate regulatory scheme where Dun1 prevent chromosome segregation during DNA damage by inhibiting Rsp5-mediated proteolytic degradation of securin Pds1.

SEPARASE antibody

70R-31054 100 ug
EUR 392.4
Description: Rabbit polyclonal SEPARASE antibody

Separase antibody

70R-51689 100 ul
EUR 344.4
Description: Purified Polyclonal Separase antibody

Separase Antibody

ABF9195 100 ug
EUR 525.6

Separase Antibody

E18-9195-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

Separase Antibody

E18-9195-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

Separase Antibody

E38PA4405 100ul
EUR 225
Description: Available in various conjugation types.

Separase Antibody

AF9195-100ul 100ul
EUR 280

Separase Antibody

AF9195-200ul 200ul
EUR 350

Separase Antibody

AF9195 100ul
EUR 280
Description: Human

SEPARASE antibody (Ser801)

70R-31053 100 ug
EUR 392.4
Description: Rabbit polyclonal SEPARASE antibody (Ser801)

Separase (pS801) Antibody

20-abx134111
  • EUR 376.80
  • EUR 560.40
  • EUR 243.60
  • 100 ul
  • 200 ul
  • 30 ul

Rabbit Polyclonal Separase Antibody

TA309645 100 µl Ask for price

Separase Conjugated Antibody

C44415 100ul
EUR 476.4

Separase Polyclonal Antibody

ABP52424-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ABP52424-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ABP52424-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Separase from Human, Mouse. This Separase antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Separase Polyclonal Antibody

ES3423-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase Polyclonal Antibody

ES3423-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Separase from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase Polyclonal Antibody

E20-74243 100ug
EUR 225
Description: Available in various conjugation types.

SEPARASE (Ab-801) Antibody

E11-0086B 100μg
EUR 225
Description: Available in various conjugation types.

SEPARASE (Ab-801) Antibody

8B0086 50ug
EUR 368
Description: SEPARASE (Ab-801) Antibody

Separase (A795) polyclonal antibody

E43P0374 100ul
EUR 225
Description: Available in various conjugation types.

SEPARASE (Phospho-Ser801) Antibody

E11-0086A 100μg
EUR 225
Description: Available in various conjugation types.

SEPARASE (Phospho-Ser801) Antibody

8A0086 50ug
EUR 368
Description: SEPARASE (Phospho-Ser801) Antibody

Mouse Monoclonal Separase Antibody (XJ11-4D7)

TA309647 100 µl Ask for price

Polyclonal ESPL1 / Separase Antibody (aa767-816)

APR02989G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ESPL1 / Separase (aa767-816). This antibody is tested and proven to work in the following applications:

Mouse Monoclonal Separase Antibody (XJ11-1B12)

TA309646 100 µl Ask for price

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ABP50519-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Separase phospho Ser801) from Human, Mouse. This Separase phospho Ser801) antibody is for WB , IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Separase around the phosphorylation site of S801

Separase (phospho Ser801) Polyclonal Antibody

ES1518-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (phospho Ser801) Polyclonal Antibody

ES1518-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Separase (phospho Ser801) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Separase (phospho Ser801) Polyclonal Antibody

E20-60872 100ug
EUR 225
Description: Available in various conjugation types.

Separase (Phospho-Ser801) Polyclonal Antibody

12353-100ul 100ul
EUR 302.4

Separase (Phospho-Ser801) Polyclonal Antibody

12353-50ul 50ul
EUR 224.4

Separase (Phospho-Ser801) Polyclonal Antibody

E012353 100μg/100μl
EUR 255
Description: Available in various conjugation types.

Separase (ESPL1) rabbit polyclonal antibody, Aff - Purified

AP32216PU-N 50 µl Ask for price

Separase (ESPL1) rabbit polyclonal antibody, Aff - Purified

AP20283PU-N 100 µg Ask for price

Separase Blocking Peptide

AF9195-BP 1mg
EUR 234

Separase Blocking Peptide

20-abx161616
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

SEPARASE Cell ELISA Kit

abx595539-96tests 96 tests
EUR 764.4

Separase (ESPL1) pSer1126 rabbit polyclonal antibody, Aff - Purified

AP12707PU-N 400 µl Ask for price

Separase (pS801) Blocking Peptide

20-abx162232
  • EUR 376.80
  • EUR 610.80
  • 1 mg
  • 5 mg

SEPARASE Cell-Based ELISA Kit

EKA50537-5x96T 5x96T
EUR 2122.78

SEPARASE Cell-Based ELISA Kit

EKA50537-96T 96T
EUR 446.9

Separase (phospho Ser801) Rabbit pAb

E2252078 100ul
EUR 225
Description: Available in various conjugation types.

Separase (ESPL1) (1866-1966) mouse monoclonal antibody, clone XJ11-1B12, Azide Free

AM26568AF-N 100 µg Ask for price

SEPARASE Colorimetric Cell-Based ELISA Kit

EKC1516 100ul
EUR 686.4

SEPARASE Colorimetric Cell-Based ELISA Kit (OKAG01028)

OKAG01028 96 Wells
EUR 715.2
Description: Description of target: ;Species reactivity: Human, Mouse;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:

SEPARASE (Phospho-Ser801) Colorimetric Cell-Based ELISA Kit

EKC2000 100ul
EUR 686.4

Anti-Separase Antibody

A04364 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Separase Antibody (ESPL1) detection. Tested with WB, IHC, IF in Human, Mouse.

Anti-Separase antibody

STJ95608 200 µl
EUR 236.4
Description: Rabbit polyclonal to Separase.

Addi platform separators (4 ea) Add 1.25 to platform separation

MIX7118 EACH
EUR 74.4

Separin (ESPL1) Antibody

20-abx212982
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Separin (ESPL1) Antibody

20-abx211834
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Separin (ESPL1) Antibody

20-abx326179
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Separin (ESPL1) Antibody

20-abx301075
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Rabbit anti-Separase Antibody

YLD7067-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to Separase

Rabbit anti-Separase Antibody

YLD7067-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to Separase

Rabbit anti-Separase Antibody

DL93405A-100ul 100 ul
EUR 299
Description: ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Rabbit anti-Separase Antibody

DL93405A-50ul 50 ul
EUR 209.3
Description: ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

SEPARIN (pS1126) Antibody

abx031912-400ul 400 ul
EUR 627.6

SEPARIN (pS1126) Antibody

abx031912-80l 80 µl
EUR 343.2

Separin (ESPL1) Antibody (HRP)

20-abx316258
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Additional platform separators (4 ea.). Adds 1.25" to platform separation

BR2000-SP 1 PC
EUR 52.8

Separator

INS6224 EACH
EUR 20.4

Separin (ESPL1) Antibody (FITC)

20-abx316259
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Separin (ESPL1) Antibody (Biotin)

20-abx316260
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Puck Separator

M-CBP-PST1 1 UNIT
EUR 340
Description: Puck Separator

Separator UB 200mm

SIE1104 EACH
EUR 126.64

Phase Separator 15ml

CHR5382 PK100
EUR 139.2

Phase Separator 25ml

CHR5384 PK100
EUR 152.76

Phase Separator 70ml

CHR5386 PK50
EUR 116.4

Phase Separator 150ml

CHR5388 PK25
EUR 145.2

Puck Separator Tools

M-CP-111-028 1 UNIT
EUR 305
Description: Puck Separator Tools

Puck Separator Tools

X-CC-116 1pc.
EUR 446.7

Mannose Separopore® 4B Cell Separation Kit

20840070-1 -
EUR 166.28

Magnetic Separator

B23803 2 mL/200 uL/15 mL
EUR 291.6
Description: This Magnetic Separator is a proprietary machine, supportinga the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time.

Magnetic Separator

MagSep1-1 1/pk
EUR 132

McDonald Separator

INS6226 EACH
EUR 16.8

Separating Gel Buffer 4X

10530008-1 100 mL
EUR 38.93

Separating Gel Buffer 4X

10530008-2 250 mL
EUR 63.78

Separating Gel Buffer 4X

10530008-3 500 mL
EUR 86.02

MiniTube Mag Separator

MTMS-16 mL
EUR 432
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Jenway Water Separator

FLA1034 EACH
EUR 184.8

Separating Funnel PP 500ml

FUN21060 PK2
EUR 93.6

50ml Separating Funnel

FUN4123 EACH
EUR 104.4

BioMag® Flask Separator

84101S-1 1each
EUR 537

Rabbit anti-Separase (pS801) Antibody

YLD7066-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to Separase (pS801)

Rabbit anti-Separase (pS801) Antibody

YLD7066-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to Separase (pS801)

Rabbit anti-Separase (pS801) Antibody

DL92370A-100ul 100 ul
EUR 299
Description: ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Rabbit anti-Separase (pS801) Antibody

DL92370A-50ul 50 ul
EUR 209.3
Description: ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Anti-Phospho-Separase (S801) antibody

STJ91032 200 µl
EUR 236.4
Description: Rabbit polyclonal to Phospho-Separase (S801).

Pyrex 1L Separating Funnel

FUN4126 EACH
EUR 139.2

Pyrex 2L Separating Funnel

FUN4128 EACH
EUR 187.2

Separatory Funnel Pear 500ml

2394T/500 PK2
EUR 244.8

Separating Funnel Pear 1000ml

FUN20650 PK2
EUR 205.2

Separating Funnel Pear 50ml

FUN2081 EACH
EUR 74.4

Separating Funnel Pear 100ml

FUN2083 EACH
EUR 85.2

Separating Funnel Pear 250ml

FUN2085 EACH
EUR 90

2L P/Shaped Separating Funnel

FUN4310 EACH
EUR 207.6

Separating Funnel Holder

STA1094 EACH
EUR 39.24

Magnetic Separation Rack

CM101 32 well (200 μl/well)
EUR 112

Magnetic Separation Rack

CM103 24 well (1.5 ml/well)
EUR 112

Separating funnel 100ML PTFE Key

8S149/100 EACH
EUR 45.6

Separating Funnel 500ml PTFE Key

8S149500 EACH
EUR 56.4

Jenway Water Separator Large

FLA1036 EACH
EUR 224.4

Pyrex 100ml Separating Funnel

FUN4120 EACH
EUR 104.4

Pyrex 250ml Separating Funnel

FUN4122 EACH
EUR 114

Pyrex 500ml Separating Funnel

FUN4124 EACH
EUR 120

Funnel Pyrex Separating Pear 5L

FUN4312 EACH
EUR 309.6

BioMag® 15ml/50ml Tube Separator

84102S-1 1each
EUR 1453

BioMag® 12 x 75mm Test Tube Separator

84104S-1 1each
EUR 1901

Cardboard Separator kit for xxxx

CRY2076 PK6
EUR 26.4

Long Form Separating Funnel 50ml

8S14950 EACH
EUR 44.4

50ml P/Shaped Separating Funnel

FUN4300 EACH
EUR 110.4

BioMag® 96-Well Plate Separator

8MB4109S-1 1magnet
EUR 955

Magnetic Separator (Re-usable)

1999-1 each
EUR 319.2

MicroMag Magnetic Separator

MMS-2100 mL
EUR 498
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

FlexiMag Magnetic Separator

FMS-1000 mL
EUR 583.2
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

FlexiMag Magnetic Separator

FMJ-1000 mL
EUR 432
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Nalgene 125ml Separating Funnel PP

FUN4180 EACH
EUR 97.2

HandiMag Magnetic Separator

HMS-1000 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

UltraMag DW Magnetic Separator

UMDS-1000 mL
EUR 741.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

UltraMag Magnetic Separator

UMS-3000 mL
EUR 736.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Separin Phospho-Ser801 (ESPL1 pS801) Antibody

20-abx326180
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Statebourne 1 2 BSP Phase Separator

CRY2104 EACH
EUR 147.53

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200070 EACH
EUR 45.6

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200110 PK100
EUR 60

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200125 PK100
EUR 68.4

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200150 PK100
EUR 96

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200185 EACH
EUR 110.4

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200240 EACH
EUR 154.8

WHATMAN(R) 1PS PHASE SEPARATOR FILTER PA

WHA2200270 EACH
EUR 238.8

T-Pro Separating or Resolving Buffer

JB03-C001 500ml/BT
EUR 182.4

EpiMag HT (96-Well) Magnetic Separator

Q10002
  • EUR 416.70
  • EUR 258.50
  • 1/Pack
  • 1/Pack

WHATMAN(R) TG100 SEPARATION GAUZE DIAM

WHA10423029 EACH
EUR 174

Culture Tube, 5mL, 12 x 75mm, PP, w/ separate 2-position screw-cap, non-sterile, non-graduated, 1000 tubes and capped bulk packed separately

T8750 1000/pack
EUR 147.48
Description: Culture Tube 5mL

Culture Tube, 14mL, 17 x 100mm, PP, w/ separate 2-position screw-cap, non-sterile, non-graduated, 1000 tubes and capped bulk packed separately

T8850 1000/pack
EUR 228.48
Description: Culture Tube 14mL

100 ML LSM (LYMPHOCYTE SEPARATION MEDIUM)

25-072-CI 100 mL/pk
EUR 63.6
Description: Media Catalog; Cell Culture Reagents

Separator type 10001 for drawers capacity 10000 cm3

DD039473 EACH
EUR 7.2

PCR 8-Strips, 0.2ml with Separate Flat Cap Strips, 120/pk

P3010-S 120/pack
EUR 116.7
Description: PCR 8-Strips 0.2ml with Separate Flat Cap Strips

Lymphocyte Separation Solution

20828-15 500ML
EUR 207.9

Lymphocyte Separation Solution

20828-44 100ML
EUR 46.2

Lymphocyte Separation Solution

20839-04 100ML
EUR 57.4

Lymphocyte separation medium (human)

abx082102-200ml 200 ml
EUR 243.6

Cytosol/Particulate Separation Kit

55R-1356 50 assays
EUR 661.2
Description: Cytosol/Particulate Separation Kit for use in the research laboratory

Cytosol/Particulate Separation Kit

K267-50 each
EUR 483.6

Cytosol/Particulate Separation Kit

K2112-50 50 assays
EUR 501.6

WHATMAN 1PS PHASE SEPARATOR FILTER PAPER

WHA2200090 EACH
EUR 49.2

BioMag® MultiSep Magnetic Separator

85200-1 1magnet
EUR 430

1-Step Polymorphs, Human Cell Separation

71-AN221725 1
EUR 238.8

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 802.8

Taq DNA Polymerase - 1000 units with separate dNTP Mix

3243d 1000units
EUR 150
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Techne Separation Membranes 200x200mm

OVE3038 PK5
EUR 181.2

Separating Gel Buffer Solution(4x) for SDS-PAGE

30651-05 500ML
EUR 56

Lymphocyte Separation Medium, Sterile

40120344-1 100 ml
EUR 25.62

Lymphocyte Separation Medium, Sterile

40120344-2 250 ml
EUR 54.64

Lymphocyte Separation Medium, Sterile

40120344-3 500 ml
EUR 90.31

Thiophilic Separopore® 4B Antibody Purification Kit

20150004-1 -
EUR 178.45

Thiophilic Separopore® 4B Antibody Purification Kit

20150004-2 -
EUR 286.01

OAAB16114-400UL - SEPARIN Antibody (Phospho-Ser1126)

OAAB16114-400UL 400ul
EUR 419

Custom drilling for BSW01 (sold separately) up to 36 holes

BLO1274 EACH
EUR 260.4

Custom drilling for BSW01 (sold separately), up to 36 holes

BSW01-DR 1 PC
EUR 195.3

Funnel Separating Conical Ptfe Stopcock 100ml

E4606 EACH
EUR 33.6

Funnel Separating Conical Ptfe Stopcock 250ml

E4607 EACH
EUR 43.2

Orion Star A211 pH Benchtop Meter Separate Sensor Kit

STARA2118 EACH
EUR 1358.4

Mercury is supplied as a separate solution (PLHG-1Y).

TCLP-100 125ML
EUR 253.2

Mercury is supplied as a separate solution (PLHG-1X).

TCLP-500 500ML
EUR 429.6

Stacking platform, small 10.5"x7.5" with flat mat (3.0"separation)

B3D-STACK 1 PC
EUR 135.8

Corning 500mL LSM (Lymphocyte Separation Medium)

25-072-CV EACH
EUR 156

Clear Glass Powder Bottle with Separate Black Cap - 15mL

BOT1200 PK140
EUR 87.6


Often resulting in prolonged mitotic apoptosis1. Abbreviated tumorigenic mitosis causing aneuploidy, but it is not clear whether it also activates apoptosis machinery2. Separase, cysteine ​​proteases and triggers of all anaphases eukaryotic, have caspase-like catalytic domains but have never been associated with cell death3,4. Here we show that human cells entering mitosis with separase already active to quickly undergo mitosis death because cleavage directly MCL1 and anti-apoptotic BCL-XL by separase. Cleavage is not only prevent MCL1 and BCL-XL from the execution of pro-apoptotic BAK, but also convert them into active promoters of death in mitosis.

Karina

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